Androgen Receptor Antibody Properties Description Anti-Androgen Receptor (MOUSE) Monoclonal Antibody - 200-301-H54 Host Mouse Target Species Human Known Cross Reactivity Human Clonality Monoclonal 156C135.2 IgG1 kappa Application Western Blot : 1-4 µg/mL Immunohistochemistry: User Optimized Physical State Liquid Shipping Condition Dry Ice Format IgG1 Label Unconjugated Concentration 0.5 mg/mL by UV absorbance at 280 nm Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Preservative 0.05% (w/v) Sodium Azide Stabilizer 0.05% BSA GeneName AR Relevant Links UniProtKB - P10275NCBI - NP_000035.2GeneID - 367

Androgen Receptor Antibody Description

Background Anti-Androgen Receptor Antibody detects human Androgen receptor. The androgen receptor (AR) is an ~110 kDa androgen-dependent transcription factor that is a member of the steroid/nuclear receptor gene superfamily. The AR signaling pathway plays a key role in development and function of male reproductive organs, including the prostate and epididymis. AR also plays a role in nonreproductive organs, such as muscle, hair follicles, and brain. Abnormalities in the AR signaling pathway have been linked to a number of diseases, including prostate cancer, Kennedys disease and male infertility. The PI3K/Akt signaling pathway plays an important role in regulating AR activity through phosphorylation of AR at Ser213/210 and Ser791/790. Growth factors or cytokines may induce phosphorylation of AR through the PI3K/Akt pathway. IGF-1 activates the phosphatidylinositol 3-kinase(PI3K)/AKT pathway in LNCap at high passage number and increases phosphorylation of of AR at Ser213/210 (see western blot) and Ser791/790. The western blot results also show that inhibition of the PI3K/Akt pathway by LY294002 prior to incubation with IGF-1 suppressed AR phosphorylation at Ser213/210. Activation of the PI3K/AKt pathway is thought to have a survival role in prostate cancer by protecting cells from apoptosis. Anti-Androgen Receptor Antibody is ideal for investigators involved in neuroscience, cytokines and growth factors, and transcription factors. Synonyms DHTR, NR3C4 Immunogen Androgen Receptor Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to amino acids near the n-terminus of the Androgen Receptor. Immunogen Type Peptide Storage Condition Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Application Note Anti-Androgen Receptor Antibody is suitbale for use in IHC (p) and WB. Expect a band approximately 110kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user. Purity/Specifity Anti-Androgen Receptor Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Anti-Androgen Receptor from human and New World Monkey on 100% homology with the immunizing sequence. Cross-reactivity with Anti-Androgen Receptor from other sources has not been determined. Disclaimer Note-General This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.