| Product Name | TNF-alpha (Low Endotoxin ) |
| Size | 10 µg |
| Description | Tumor necrosis factor alpha (TNFα or TNFSF2) is produced by many different cell types including activated monocytes, fibroblasts, endothelial cells, macrophages, T-cells, B-lymphocytes, granulocytes, smooth muscle cells, eosinophilis, chondrocytes and other cell types (1). It functions as a cytokine involved in cell proliferation, differentiation, apoptosis, lipid metabolism and coagulation (2, 3). High level of TNFα leads to the development of inflammatory responses that are hallmarks of many diseases including autoimmune diseases, insulin resistance and cancer (4, 5). The active form of TNFa (18 kDa) is generated by the cleavage of an Ala-Val bond between residues 76-77 of the precursor protein (6). |
| Applications | cytotoxicity assays and other cell assays |
| Source | E. coli |
| Fusion Tag | 6X His |
| Purification Method | FPLC |
| Unit Definition (Activity) | 1 unit equals to 1 nanogram of purified protein. |
| Purity | ~ 95% as determined by SDS-PAGE |
| Gene ID | 7124 |
| Accession # | NM_000594 |
| Gene Name | tumor necrosis factor |
| Gene Symbol | TNF |
| Gene Synonyms | DIF; TNFA; TNFSF2; TNF-alpha |
| MW | 21 kDa |
| Protein Sequence | VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYSQVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYLGGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL |
| Storage Buffer | 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol |
| Storage | -20 to -80 °C. |
| Shipping | 4°C or dry ice |
| References | 1. Locksley, R. et al., (2001) Cell 104, 487-5012. Mukhopadhyay, S. et al., (2006) Respiratory Res. 7, 1-93. Aggarwal, B. (2003) Nat. Rev. Immunol. 3, 745-7564. Walczak, H. et al., (1999) Nat. Med. 5, 157-1635. Lam, J. et al., (2000) J. Clin. Invest. 106, 1481-14886. Mcgeehan, G. et al., (1994) Nature 370, 558-561 |
