Enzolifesciences/MDA5 polyclonal antibody/ENZ-ABS299-0100/100µg
Product Specification:
| Alternative Name: | Helicard, Melanoma differentiation-associated gene 5, Interferon-induced helicase C domain-containing protein 1, RH116, CADM-140 autoantigen, RIG-I-like receptor 2, RLR-2 |
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| Host: | Rabbit |
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| Isotype: | IgG |
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| Immunogen: | Synthetic peptide corresponding to 16 amino acids from near the center of human MDA5. |
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| UniProt ID: | Q9BYX4 (human), Q8R5F7 (mouse) |
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| Species reactivity: | Human, Mouse |
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| Applications: | ELISA, IF, IHC (PS), WB |
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| Recommended Dilutions/Conditions: | Immunofluorescence (10µg/ml)Immunohistochemistry (5µg/ml)Western Blot (1-4µg/ml)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. |
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| Purity Detail: | Affinity purified. |
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| Formulation: | Liquid. In PBS containing 0.02% sodium azide. |
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| Use/Stability: | Stable for at least 1 year after receipt when stored as recommended. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Shipped on Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Scientific Background: | MDA5 is a highly conserved helicase whose ATPase activity can be stimulated by RNA and less potently by DNA. It contains two N-terminal CARD domains and a C-terminal helicase domain. The helicase domain, once freed from the CARD domains, then translocates from the cytoplasm to the nucleus where it acts on chromatin architecture. This could allow a more easy access of CAD, thereby accelerating the degradation of genomic DNA. |
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