APC6 phospho T580 Antibody Properties Description Anti-APC6 pT580 (RABBIT) Antibody - 600-401-876 Host Rabbit Target Species Human Known Cross Reactivity human Clonality Polyclonal Application ELISA : 1:1,000 - 1:10,000 IF Microscopy : User Optimized Western Blot : 1:500 - 1:3,000 Other Dilution: User Optimized Physical State Liquid (sterile filtered) Shipping Condition Dry Ice Format IgG Label Unconjugated Concentration 0.97 mg/mL by UV absorbance at 280 nm Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Preservative 0.01% (w/v) Sodium Azide Stabilizer None GeneName CDC16 Relevant Links NCBI - 37537763UniProtKB - Q13042GeneID - 8881

APC6 phospho T580 Antibody Description

Background APC6 (also known as Cell division cycle protein 16 homolog (CDC16Hs), anaphase promoting complex subunit 6, and cyclosome subunit 6) is a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis and the G₁ phase of the cell cycle.   The APC/C is composed of at least 11 subunits.   APC6 interacts with PPP5C and CDC20. This protein colocalizes with CDC27 to the centrosome at all stages of the cell cycle and to the mitotic spindle.  Splice variants have been described for this protein. Synonyms rabbit anti-APC6 pT580 Antibody, APC 6, APC-6, Anaphase promoting complex subunit 6 antibody, CDC16Hs antibody, Cell division cycle 16 homolog antibody, Cell division cycle protein 16 homolog antibody, Cyclosome subunit 6 antibody Immunogen This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 575-588 of Human Apc6 protein. Immunogen Type Peptide Post Translational Modification Phosphorylation Storage Condition Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Application Note This affinity purified antibody has been tested for use in ELISA and western blot.   Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 72 kDa, 71.5 kDa or 65.9 kDa in size corresponding to isoforms of APC6 by western blotting in the appropriate cell lysate or extract. Purity/Specifity This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. This antibody is specific for phosphorylated human APC6 protein at the pT580 residue. BLAST analysis indicates 100 % homology of the immunizing sequence with APC6 from human.  No cross reactivity is expected against homologues from other sources.  This antibody is expected to react with all isoforms of this protein.  Minimal reactivity is expected with the non-phosphorylated form of the protein. Disclaimer Note-General This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.