Details
Pvu I Source
Proteus vulgaris
Reagents Supplied
CMX Buffer 3
Unit Definition
One unit is the amount of enzyme required to completely digest 1 µg of Adenovirus 2 DNA in 1 hour at 37°C in a total reaction volume of 50 µl.
Star Activity
No
Incubation Temperature
37°C
Heat Inactivation
10 minutes at 80°C
Assay Unit Substrate
Adenovirus 2 DNA
Reaction Buffer
CMX Buffer 3;50 mM Tris-HCl (pH 7.4 at 37°C)10 mM MgCl2100 mM NaCl21 mM dithiothreitol100 µg/ml bovine serum albumin
Storage Buffer
50mM Tris-HCl (pH 7.5 at 4°C)200 mM KCl 0.1 mM EDTA10mM β-mercaptoethanol 0.2% (v/v) Triton X-100™500 µg/ml bovine serum albumin 50% (v/v) glycerol
Assay Conditions
50 mM Tris-HCl (pH 7.5 at 37°C)10 mM MgCl2, 100 mM NaCl1 mM dithiothreitol100 µg/ml bovine serum albumin1 µg Adenovirus 2 DNAReaction volume 50 µl
Storage Conditions
Store at -20°CShipped on dry ice
Pvu I Downloads
Certificate of Analysis PDF-Current LotMSDS PDF-Current Lot
Categories
Restriction Endonucleases, Pvu I
