Source: E. coliContent: 10 µg
Overview
BMP-2 stands for bone morphogenetic protein 2. Human BMP-2 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.
Applications
Human BMP-2 can be used for a variety of applications, including:
- Study of chondrogenesis and osteogenesis.
- Differentiation of mesenchymal stem cells into several cell type including osteoblast and cardiac cells.
- Investigation of TGF-β signaling pathway.
Details
Background information
Bone morphogenetic protein 2 (BMP-2) is a member of the bone morphogenetic protein family, which is part of the TGF-β superfamily. Together with BMP-7, BMP-2 is a potent stimulator of osteoblast differentiation as well as of chondrocyte proliferation of mesenchymal cells during bone and cartilage formation, and can also induce cardiac and epithelial morphogenesis. BMP-2 is secreted by several cell tissues such as spleen, lung, liver, brain, small intestine, prostate, and ovary, as immature long form, and after proteolytic cleavage it matures into an active disulfide-linked homodimer. BMP-2 signals through heterodimeric receptors, such as BMPR1A, and activates TGF-β signaling cascade and the hedgehog pathway. Besides the morphogenic role, BMP-2 is also crucial during embryonic dorsal-ventral generation and organogenesis. Recombinant BMP-2 is under clinical investigation for treatment of bone degeneration diseases, spinal injuries, and orthodontic pathologies. Mature human mouse and rat BMP-2 share full amino acid sequence identity. Recombinant Human BMP-2 corresponds to the mature form of BMP-2.
Biological activity
Alkaline phosphatase assay using mouse C2C12 fibroblasts (NIBSC 93/574)research grade: ≥ 5×10
4 U/mgpremium grade: ≥ 1×10
5 U/mg
Quality description
Research-grade cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
