Description:

INTENDED USE The GenWay, Inc. Cortisol ELISA kit is used for the quantitative measurement of Cortisol in sheep serum orplasma.

SUMMARY AND EXPLANATION Cortisol (hydrocortisone, compound F) is the most potent glucocorticoid synthesized from cholesterol. Cortisol isfound the blood either as free Cortisol, or bound to corticosteroid-binding globulin (CBG). Cortisol productionhas an ACTHdependent circadian rhythm with peak levels in the early morning and a nadir at night. The factorscontrolling this circadian rhythm are not completely defined. Serum levels are highest in the early morning anddecrease throughout the day. In the metabolic aspect, Cortisol promotes gluconeogenesis, liver glycogendeposition, and the reduction of glucose utilization. Immunologically, Cortisol functions as an important antiinflammatory, and plays a role in hypersensitivity, immunosuppression, and disease resistance. This kit can detect low level of cortisol in sheep serum or plasma (less than 0.1 ng/ml).

PRINCIPLE OF THE TESTThe CBI Cortisol is a solid phase competitive ELISA. The samples and Cortisol enzyme conjugate are added tothe wells coated with anti-Cortisol monoclonal antibody. Cortisol in the patient’s sample competes with aCortisol enzyme conjugate for binding sites. Unbound Cortisol and Cortisol enzyme conjugate is washed off bywashing buffer. Upon the addition of the substrate, the intensity of color is inversely proportional to theconcentration of Cortisol in the samples. A standard curve is prepared relating color intensity to theconcentration of the Cortisol.

SENSITIVITYThis assay sensitivity was found to be 0.1ng/ml. The sensitivity was determined by calculating the mean plus2SD of the standard zero point tested 20 times in the same run.

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