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Tim Koppen

Center for Biochemistry, Medical Faculty, University of Cologne, Köln, Germany

Search for more papers by this author Andreas Weckmann

Center for Biochemistry, Medical Faculty, University of Cologne, Köln, Germany

Search for more papers by this author Stefan Müller

Central Bioanalytics, Center for Molecular Medicine Cologne, University of Cologne, Köln, Germany

Search for more papers by this author Simon Staubach

Center for Biochemistry, Medical Faculty, University of Cologne, Köln, Germany

Search for more papers by this author Wilhelm Bloch

Department of Molecular and Cellular Sport Medicine, German Sport University Cologne, Köln, Germany

Search for more papers by this author R. Jürgen Dohmen

Institute for Genetics, University of Cologne, Köln, Germany

Search for more papers by this author Tilo Schwientek

Corresponding Author

E-mail address: akd88@uni‐koeln.de

Center for Biochemistry, Medical Faculty, University of Cologne, Köln, Germany

Institut für Biochemie II, Medizinische Fakultät der Universität Köln, Joseph‐Stelzmann‐Strasse 52, D‐50931 Köln, Germany Fax: +49‐221‐4786977===Search for more papers by this author Tim Koppen

Center for Biochemistry, Medical Faculty, University of Cologne, Köln, Germany

Search for more papers by this author Andreas Weckmann

Center for Biochemistry, Medical Faculty, University of Cologne, Köln, Germany

Search for more papers by this author Stefan Müller

Central Bioanalytics, Center for Molecular Medicine Cologne, University of Cologne, Köln, Germany

Search for more papers by this author Simon Staubach

Center for Biochemistry, Medical Faculty, University of Cologne, Köln, Germany

Search for more papers by this author Wilhelm Bloch

Department of Molecular and Cellular Sport Medicine, German Sport University Cologne, Köln, Germany

Search for more papers by this author R. Jürgen Dohmen

Institute for Genetics, University of Cologne, Köln, Germany

Search for more papers by this author Tilo Schwientek

Corresponding Author

E-mail address: akd88@uni‐koeln.de

Center for Biochemistry, Medical Faculty, University of Cologne, Köln, Germany

Institut für Biochemie II, Medizinische Fakultät der Universität Köln, Joseph‐Stelzmann‐Strasse 52, D‐50931 Köln, Germany Fax: +49‐221‐4786977===Search for more papers by this author

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Distinct types of vesicles are formed in eukaryotic cells that conduct a variable set of functions depending on their origin. One subtype designated circulating microvesicles (MVs) provides a novel form of intercellular communication and recent work suggested the release and uptake of morphogens in vesicles by Drosophila cells. In this study, we have examined cells of the hemocyte‐like cell lines Kc167 and S2 and identified secreted vesicles in the culture supernatant. The vesicles were isolated and found to have characteristics comparable to exosomes and plasma membrane MVs released by mammalian cells. In wingless‐transfected cells, the full‐length protein was detected in the vesicle isolates. Proteomics analyses of the vesicles identified 269 proteins that include various orthologs of marker proteins and proteins with putative functions in vesicle formation and release. Analogous to their mammalian counterparts, the subcellular origin of the vesicular constituents of both cell lines is dominated by membrane‐associated and cytosolic proteins with functions that are consistent with their localization in MVs. The analyses revealed a significant overlap of the Kc167 and S2 vesicle proteomes and confirmed a close correlation with non‐mammalian and mammalian exosomes.

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