product information Background SnRK2.2, SnRK2.3, SnRK2.6 are members of SNF1-related protein kinases whose activity is activated by ionic (salt) and non-ionic (mannitol) osmotic stress. Involved in ABA signalling pathway. Alternative names: OST1-kinase-like 2, Protein ATHPROKIN B, SNF1-related kinase 2.3, SnRK2.3, Protein OPEN STOMATA 1SNF1-related kinase 2.6, SnRK2.6, Serine/threonine-protein kinase OST1, OST1-kinase-like 3, Protein ATHPROKIN A, SNF1-related kinase 2.2, SnRK2.2 Immunogen KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SnRK2.2 UniProt:Q39192 ,TAIR: At3g50500, SnRK2.3, UniProt: Q39193 TAIR: At5g66880, SnRK2.6 UniProt: Q940H6, TAIR: At4g33950 Host Rabbit Clonality Polyclonal Clone Purity Affinity purified serum Format Lyophilized in PBS pH 7.4 Quantity 50 µg Reconstitution For reconstitution add 50 µl of sterile water. Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Tested applications Western Blot (WB) Related products AS13 2635 | SRK2E | Ser/Thr-protein kinase SnRK2.6 AS12 1855 | anti-S6K1/2 | Ribosomal S6 kinase 1/2, rabbit antibodiesAS13 2664 | anti-S6K1-2 | Ribosomal-protein S6 kinase homolog 1,2 - phosphorylated, rabbit antibodies Plant protein extraction buffer Secondary antibodies Additional information

application information
Recommended dilution		1 : 1000 (WB)
Expected | apparent MW		41 kDa
Confirmed reactivity		Arabidopsis thaliana
Predicted reactivity		Brassica oleracea, Glycine max, Medicago sativa, Morsu notabilis, Oryza sativa, Phaseolus vulgaris, Populus sp., Triticum sp., Vicia faba
Not reactive in		Chlamydomonas reinhardtii
Additional information		to be added when available
Selected references		to be added when available, antibody released in September 2015

application example

20 µg of total protein from two-week-old seedling of Arabidopsis thaliana Col-0 (1), snrk2.2/3/6 (2), snrk1/4/5/7/8/9/10 (3) extracted with kinase buffer were separated on 10 % SDS-PAGE and blotted 30 min to PVDF using semi-dry. Blots were blocked with 5% skim milk in TBST for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5000 in 0.5% TBST for overnight at 4 oC with agitation. The antibody solution was decanted and the blot was washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG goat peroxidase conjugated, from Bio-Rad) diluted to 1: 5 000 in 0.5 % TBST for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 30 seconds.Dr. Pengcheng Wang, Purdue University, USA

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