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Agrisera/UBQ11 | Ubiquitin/AS08 307/
product information
Background

Ubiquitin is a highly conserved regulatory protein expressed in all eukaryotic tissues. Originally this protein was called: Ubiquitous Immunopoietic Polypeptide. Its function is labeling of proteins for degradation through ubiquitin proteasome system (UPS).

Immunogen

Recombinant Arabidopsis thaliana ubq11, with N-terminal His-tag; UniProt:P0CH33, TAIR: AT4G05050

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water
Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)
Related products AS10 307A | antt-UBQ11 | Ubiquitin, affinity purified, rabbit antibody

AS10 307S | UBQ11 | Ubiquitin protein | positive control

Additional information
application information
Recommended dilution

1: 10 000 (WB)

Expected | apparent MW

8.5 kDa

Confirmed reactivity Arabidopsis thaliana, Hordeum vulgare, Nicotiana benthamina, Solanum lycopersicum
Predicted reactivity

dicots including: Nicotiana tabacum, Glycine max, Glycine soja, Pisum sativum, Phaseolus vulgaris, Capsella rubella, Medicago truncatula, Vitis vinifera monocots including: Oryza sativa, Zea mays   Brassica napus, Medicago truncatula, Erythrane guttata, Brachypodium distachyon, Triticum aestivum, Cochliobolus heterostrophus, Solanum tuberosum, Solanum nigrum, Ipomoea batatas, Populus trichocarpa, Capsella rubella, Cymbidium faberi, Magnaporthe oryzae, Pyrus communis, Citrus sinensis, Citrus clementina, Eucalyptus grandis, Sorghum bicolor, Nicotiana tabacum, Ricinus communis, Theobroma cacao, Coffea canephora, Prunus persica,

Not reactive in

algae

Additional information

antibody can be used to check qubiquitination status in the whole plant extracts.

Selected references Witzel et al. (2017). A Proteomic Approach Suggests Unbalanced Proteasome Functioning Induced by the Growth-Promoting Bacterium Kosakonia radicincitans in Arabidopsis. Front Plant Sci. 2017 Apr 26;8:661. doi: 10.3389/fpls.2017.00661. Gorovits et al. (2017). The six Tomato yellow leaf curl virus genes expressed individually in tomato induce different levels of plant stress response attenuation. Cell Stress Chaperones. 2017 Mar 21. doi: 10.1007/s12192-017-0766-0. Moshe at al. (2015). Tomato plant cell death induced by inhibition of HSP90 is alleviated by Tomato yellow leaf curl virus infection. Mol Plant Pathol. 2015 May 12. doi: 10.1111/mpp.12275. Hamorsky et al. (2015). N-Glycosylation of cholera toxin B subunit in Nicotiana benthamiana: impacts on host stress response, production yield and vaccine potential. Sci Rep. 2015 Jan 23;5:8003. doi: 10.1038/srep08003. Kong et al. (2014). Quantitative proteomics analysis reveals that the nuclear cap-binding complex proteins Arabidopsis CBP20 and CBP80 modulate the salt stress response. J Proteome Res. 2014 Apr 1. Zulet et al. (2013). Proteolytic Pathways Induced by Herbicides That Inhibit Amino Acid Biosynthesis. PLoS ONE 8(9): e73847. doi:10.1371/journal.pone.0073847. (Pisum sativum, western blot)Ferrández-Ayela et al. (2013). Arabidopsis TRANSCURVATA1 Encodes NUP58, a Component of the Nucleopore Central Channel. PLOS ONE, June 2013. Ustun et al. (2013). The Xanthomonas campestris Type III Effector XopJ Targets the Host Cell Proteasome to Suppress Salicylic-Acid Mediated Plant Defence. PLOS Pathog. June 9.

application example

 

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