;
王洪一
;
姚尧
;
刘晶晶
;
徐潇
;
张宇
;
刘洋
;
吴祖泽
;
靳继德
【期刊名称】
《中国组织工程研究》
【年
(
卷
),
期】
2014(000)010
【摘要】
BACKGROUND:Human
umbilical
cord
mesenchymal
stem
cells
with
capabilities
for
self-renewal
and
multi-differentiation
have
attracted
widespread
attention.
OBJECTIVE:To
develop
an
efficient
methodforisolationandcultureofhumanumbilicalcordmesenchymal
stem
cells,
and
to
analyze
the
cellbiological
features.
METHODS:Mesenchymal
stem
cells
were
isolated
and
cultured
from
human
umbilical
cord
by
improved
tissue
cultivation.
Immunophenotype
and
cellcycle
were
analyzed
by
flow
cytometry.
GrowthcurvewasdeterminedbyMTTassay,anddifferentiationability
wasevaluatedbyinvitroosteogenicandadipogenicinductionaswel.
RESULTS
AND
CONCLUSION:Some
fusiform
cells
crawled
out
from
human
umbilical
cord
tissues
after
cultivation
for
5
days
and
formed
colonies
about
10
days
later.
When
the
removed
tissues
were
further
cultured,morecellsappearedagainwithin2daysandformedcolonies
after
5
days.
The
isolated
cells
exhibited
similar
morphology
of
fibroblast-like
shape
after
passage.
Furthermore,
the
cells
expressed
CD90,CD105,butwerenegativeforthemarkersofCD34,CD45,HLA-
DR.Populationdoublingtimeofthecellscalculatedfromtheresultof
