Interact Reference Component Highly efficient Applied Biosystems real-time PCR solutions minimize complexities to help maximize your time and effort
Real-time PCR, also known as quantitative PCR (qPCR), is the gold-standard for sensitive, specific detection and quantification of nucleic acid targets. We developed powerful assay design algorithms, optimized master mixes, created intuitive data analysis software, and built smart instrumentation to help harness the power of real-time PCR across a rich and diverse set of applications. However, our solutions go a step further: every product has been designed with efficiency baked in. And together they work synergistically to bring you a simpler, more flexible, and highly efficient real-time PCR workflow.
Elements of the Applied Biosystems real-time PCR ecosystem TaqMan Real-time PCR assaysIndustry-leading choice for 5ʹ nuclease real-time PCR assays. 20 million predesigned assays (primer/probe sets) available in five formats and unlimited content configurations. Custom designs and individual primers/probes also available.
TaqMan Gene Expression AssaysTaqMan Array cards platesTaqMan primers probesView all TaqMan Assays
QuantStudio Real-time PCR systemsWith more than 25 years of qPCR innovation, Applied Biosystems continues to lead the way in performance, reliability, and world-class support. From low- to high-throughput solutions, there’s a QuantStudio real-time PCR system that’s just right for your research.
QuantStudio system overviewRequest a virtual demoRequest more information or a quoteView all QuantStudio systems
Real-time PCR reagents kitsTaqMan master mixes are optimized to work with our TaqMan Assays to provide optimal sensitivity, specificity, and dynamic range. Our SYBR portfolio offers a variety of features providing a wide range of instrument compatibility.
TaqMan real-time PCR master mixesSYBR Green real-time PCR master mixesRequest a sampleView all reagents kits
Real-time PCR data analysisFrom gene expression to Ct analysis and copy number variation determination, we have developed robust analysis software for nearly every real-time PCR application. Plus, all our analysis tools have been optimized for use with our TaqMan Assays on Applied Biosystems Real-time PCR instruments.
qPCR analysis modulesGene expression analysisConnect cloud-based platformView all data analysis software
Featured real-time PCR solutionsPowerTrack SYBR Green Master Mix
Two-color tracking dye system indicates where pipetting has occurred, reducing potential errors.
Video: Get better data with PowerTrack SYBR Green Master Mix
QuantStudio 6 7 Pro systems
Contemporary technologies for fewer touchpoints and remote monitoring in a smaller footprint.
Pre-spotted 384-well TaqMan Array cards
Can you imagine loading a 384-well array in just a few minutes? We did. Then we made it happen. Discover a simplified real-time PCR benchtop workflow with TaqMan Array cards.
Looking to commercialize? Contact our OEM services team today ›
Real-time PCR applications Gene expression (mRNA) analysisGene expression is the most common application for real-time PCR due to its efficiency at detecting and quantifying target genes, even at very low expression levels.
MicroRNA (miRNA) analysismiRNAs are small, noncoding RNA molecules that direct post-transcriptional suppression of gene expression. Our solutions enable miRNA research from isolation through discovery, profiling, quantitation, verification, and functional analysis.
Protein expression analysisTaqMan Protein assays combine the sensitive, specific protein-binding capabilities of antibodies with the superior relative quantitation capabilities of 5\' nuclease real-time PCR. They enable fast, easy identification and relative quantification of proteins from small sample sizes or limited numbers of cells—all in a homogeneous assay format.
Genetic variation analysisGenetic variation refers to the differences in the DNA sequence encoded within our genomes. Specific applications include:
SNP genotypingDrug metabolism enzyme (DME) genotypingCopy number variation (CNV) analysisMutation detectionReal-time PCR is a widely used technology for mutation detection that not only provides excellent sensitivity and specificity when paired with our gold-standard TaqMan Assays, but also provides rapid results and is easy to perform when compared to other methods. Explore these solutions:
castPCR technology for detecting somatic mutations for cancer researchFTR gene mutation analysisBiomarker analysisProminent real-time PCR disease research areas
Virology research
Detect specific viruses and verify targets involved in the immunological response to viral infection, including SARS-CoV-2 infection.
Immunology research
Detect and quantitate the expression levels of common and novel immunology biomarkers identified in transcriptomic studies.
Cancer research
Discover and verify oncology biomarkers with gold-standard specificity and sensitivity.
Immuno-oncology research
Discover and verify genomic biomarkers of well-understood checkpoint inhibitors and other immune-related genes.
Verification of NGS and Microarray Results using qPCR and TaqMan Gene Expression Assays
Publish Real-Time PCR Results with Confidence – TaqMan Assays Comply with MIQE GuidelinesReal-time PCR learning & support
Real-Time PCR Learning Center
Whether you are new to real-time PCR or an experienced user looking to learn new tricks, we have a library of educational material to help you.
Real-time PCR application support
One destination for all your application support needs: technical information, tips and tricks when starting an experiment, and answers to everyday qPCR problems.
Real-time PCR service support
Instrument service plans and support, qualification and validation services, expert training, and more. More than 1,400 trained professionals ready to help you.
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Resources Real-time vs. traditional vs. digital PCRAbsolute vs. relative quantification for qPCRTaqMan vs. SYBR Green for qPCRTaqMan Gene Expression Assays brochureApplied Biosystems on FacebookReal-Time PCR SolutionsGet the latest news from Behind the Bench blogAsk TaqMan video seriesReal-Time PCR Handbook Support Real-Time PCR troubleshooting tool仅供研究使用，不可应用于诊断程序。
