HeLa , MDA-MB-231 , U87-MG , SK-N-SH , SK-N-AS , 293T transfected with LOXL2 , mouse lungMouse reactivity has been validated by IHC-P only, not yet been tested by other applications. Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4 C. For long-term storage, aliquot and store at -20 C or below. Avoid multiple freeze-thaw cycles. Recombinant protein encompassing a sequence within the center region of human LOXL2. The exact sequence is proprietary. This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. [provided by RefSeq] LOXL2 antibody detects LOXL2 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: A431 cells were fixed in ice-cold MeOH for 5 min.Green: LOXL2 protein stained by LOXL2 antibody (GTX105085) diluted at 1:500..Blue: Hoechst 33342 staining.Scale bar = 10 m. Non-transfected (–) and transfected (+) 293T whole cell extracts (30 g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GTX105085) diluted at 1:5000. Various whole cell extracts (30 g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GTX105085) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. LOXL2 antibody detects LOXL2 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human esophageal carcinoma.LOXL2 stained by LOXL2 antibody (GTX105085) diluted at 1:3000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min LOXL2 antibody detects LOXL2 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse ovary.LOXL2 stained by LOXL2 antibody (GTX105085) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min LOXL2 antibody detects LOXL2 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human ovarian cancer.LOXL2 stained by LOXL2 antibody (GTX105085) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min LOXL2 antibody detects LOXL2 protein by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: LOXL2 stained by LOXL2 antibody (GTX105085) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar= 10 m. Wild-type (WT) and LOXL2 knockout (KO) HeLa cell extracts (30 g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GTX105085) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Various whole cell extracts (30 g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GTX105085) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. The observed M.W. is based on the publication: PMID: 21732535 The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 28273952 The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 28273952 The data was peer reviewed and published in the journal J Exp Clin Cancer Res in 2017.PMID: 28449718 The data was peer reviewed and published in the journal J Exp Clin Cancer Res in 2017.PMID: 28449718 The data was peer reviewed and published in the journal J Exp Clin Cancer Res in 2017.PMID: 28449718 The data was peer reviewed and published in the journal Oncogenesis in 2019.PMID: 31086173 The data was peer reviewed and published in the journal Oncogenesis in 2019.PMID: 31086173 The data was peer reviewed and published in the journal Front Oncol in 2020.PMID: 32211324 The data was peer reviewed and published in the journal Front Oncol in 2020.PMID: 32211324 (全国热线) 4006-800-892 / (深圳) 0755-26755892 / (北京) 010-88594029 / (上海) 021-34613729 / (广州) 18024516375 / (香港) +852-69410778