说明： Far red dye (Reactive group: NHS ester). Applications: super resolution microscopy e.g. dSTORM STED, confocal and live cell imaging别名： JF 669, NHS化学名： 1-[7-(1-Azetidinyl)-10-[2-Carboxy-5-[(2-(2,5-dioxopyrrolidin-1-yl)oxy)-2-oxoethyl]thio-3,4,6-trifluorophenyl]-9,9-dimethyl-9-silaanthracen-2(9H)-ylidene]azetidinium, inner salt 生物活性

Red fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag and SNAP-tag . Suitable for confocal fluorescent imaging and super resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells). Cell permeable. Excitation maximum = 669 nm; emission maximum = 682 nm; Quantum yield = 0.37; Extinction coefficient = 116,000 M-1cm-1; Correction factor = 0.0430. Retains 97% fluorescence after 30 bleaching cycles.

We also offer Janelia Fluor conjugated antibodies and custom conjugation services with our sister company Novus Biologicals.

HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.

许可信息

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Application of Janelia Fluor 669, SE dye on Tubulin

Application of Janelia Fluor 669 SE dye on Tubulin; Left: Microtubules were labeled with anti- -tubulin primary antibodies. The secondary antibodies were conjugated to JF 669. Maximum intensity projection of confocal z-stack recorded with an Olympus FV1200 confocal microscope using 638 nm laser line forJF 669. Right: Enlarged portion of left-hand image.

Images kindly provided by Dr Kirstin Elgass Monash University

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. C[Si](C1=CC(N2CCC2)=CC=C1C(C3=C(F)C(SCC(ON4C(CCC4=O)=O)=O)=C(F)C(F)=C3C([O-])=O)=C5C=C/6)(C)C5=CC6=[N+]7CCC\\7

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

以下数据基于产品分子量 693.77。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions. Molarity Calculator Calculate the mass, volume, or concentration required for a solution.

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Janelia Fluor 669, SE 实验方案

以下实验方案包含使用 Janelia Fluor 669, SE（目录编号 6420）的更多信息。

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Janelia Fluor 646, Azide Cat. No. 7088 Red dye (Reactive group: azide). Applications: super resolution microscopy e.g. dSTORM & STED, confocal, and live cell imaging Janelia Fluor 646, free acid Cat. No. 6993 Red dye (Reactive group: free acid). Applications: super resolution microscopy e.g. dSTORM & STED, confocal, flow and live cell imaging

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