EBV Dextramer Package 1-PE

Product Code: RX05-PE

Package of 4 carefully selected EBV specific MHC I Dextramers (+ 1 control), for sensitive and specific detection of antigen-specific T cells using flow cytometry. Each mulitmer is PE labeled.Amount: 5 x 50 testsApplication(s): Cellular immune response, Immune monitoring, T-cell assaysIndication(s)/Topic(s): Infection, Cancer, Hodgkins lymphoma, Burkitts lymphoma, Nasopharyngeal carcinoma, Dermatomyositis, Systemic lupus erythematosus, Rheumatoid arthritis, Sjgrens syndrome, Multiple sclerosisDelivery Time: 10-12 days

MHC I Dextramer CollectionsMHC I Dextramers combine a dextran polymer backbone with a high number of MHC and fluorochrome molecules providing mulitple binding domains for antigen-specific T cell receptor binding. This enhances their staining intensity and increases resolution as well as signal-to-noise ratio with minimal background staining. In addition, the dextran backbone stabilizes the MHC-peptide complexes and fluorochromes. The EBV set is useful for enumeration of EBV-specific CD8+ T cells, e.g. for immune monitoring in immune-compromised individuals following transplantation, testing of virus-specific vaccines or profiling of virus-specific T cell responses. The Dextramers are provided as PE conjugates. The following 5 Dextramers are provided with PE labelDextramer: HLA-A*0201 / GLCTLVAML - Antigen: BMLF-1Dextramer: HLA-B*3501 / HPVGEADYFEY - Antigen: EBNA-1Dextramer: HLA-B*0702 / RPPIFIRRL - Antigen: EBNA-3ADextramer: HLA-B*0801 / FLRGRAYGL - Antigen: EBNA-3ADextramer: General / Negative Control

Benefits of MHC I Dextramers®- More MHC molecules and more fluorochromes than conventional MHC multimers- Higher sensitivity (important for low-affinity epitopes as commonly found in cancer & autoimmune disease)- High stability- Custom-made Dextramers available upon request

EBV Dextramer Package 1-PE

Selected References for MHC I Dextramers®\"Combination Immunotherapy after ASCT for Multiple Myeloma Using MAGE-A3/Poly-ICLC Immunizations Followed by Adoptive Transfer of Vaccine-Primed and Costimulated Autologous T Cells\"Rapoport et al., Clin Cancer Res (2014) - PMID: 24520093 \"Molecular Imprint of Exposure to Naturally Occurring Genetic Variants of Human Cytomegalovirus on the T cell Repertoire\" Smith et al., Sci Rep (2014) - PMID: 24509977\"Probing the effector and suppressive functions of human T cell subsets using antigen-specific engineered T cell receptors\"Wan et al., PLoS One (2013) Full text \"Human Natural Killer Cells Prevent Infectious Mononucleosis Features by Targeting Lytic Epstein-Barr Virus Infection\" Chijioke et al., Cell Rep (2013) - PMID: 24360958

EBV Dextramer Package 1-PE