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Phosphorylation Site-Detector PDK1

Product Code: PD-048

Peptide microarray displaying 182 peptides derived from a peptide scan (13 mers with 10 aa overlap) through PDK1(Swiss-Prot ID: O15530) of Homo sapiens (Human).Amount: 1 peptide microarray(each peptide is deposited 3x per subarray, 4 subarrays per slide) + dummy slide and spacers (needed for incubation)Purity: Free of truncated sequencesDelivery Format: Peptides immobilized onto glass slide (3 x 1 inches; 75 by 25 mm)Application(s): Phosphosite detection, Substrate identificationIndication(s)/Topic(s): KinaseDelivery Time: 10-12 days

Phosphorylation Site-DetectorsJPT Peptide Technologies applies its unique peptide microarray platform to generate peptide microarrays on glass slides enabling characterization of enzymes such as kinases as well as methyl, glycosyl, acetyl transferases. Peptides are immobilized on glass slides via a flexible linker. Chemoselective coupling generates microarrays displaying covalently attached peptides in four identical subarrays (3 copies of peptide within each subarray). Incubation can be performed manually or automated.

Benefits of JPTs Phosphorylation Site-Detector- Get hundreds of identical microarray copies - Peptides are free of truncated sequences - Small amounts of your enzyme preparation is needed for incubation - Applicable to a variety of biological samples (e.g. serum, lysate, kinase) - Low background on glass surface - Read-out using fluorescenctly labeled antibodies or radioisotopically labeled ATP - Screen thousands of potential peptide substrates economically

Phosphorylation Site-Detector PDK1

Selected References \"Parvulin 17-Catalyzed Tubulin Polymerization is Regulated by Calmodulin in a Calcium-Dependent Manner\"Burgardt et al, J Biol Chem. (2015) - PMID: 25940090 \"Phosphopeptide Detection with Biotin-Labeled Phos-tag\"Kinoshita-Kikuta et al., Methods in Molecular Biology (2016) - PMID: 26584916\"PKc-theta is a Novel sc35 Splicing Factor Regulator in Response to T cell Activation\" McCuaig et al., Frontiers in Immunology - PMID: n.a.\"Phos-tag Technology for Kinomics\" Kinoshita-Kikuta et al., Kinomics: Approaches and Applications (2015) - PMID: n.a.\"Characterization of the Host Response to Pichinde Virus Infection in the Syrian Golden Hamster by Species-Specific Kinome Analysis\" Falcinelli et al., Mol. Cell. Proteomics. (2015) - PMID: 25573744\"Antiviral Potential of ERK/MAPK and PI3K/AKT/mTOR Signaling Modulation for Middle East Respiratory Syndrome Coronavirus Infection as Identified by Temporal Kinome Analysis\" Kindrachuk et al., Antimicrob Agents Chemother. (2015) - PMID: 25487801

Application Notes for Enzyme Substrate Microarrays\"Exploring the human acetylome using high-density peptide microarrays\"Masch et al., Application Note (2012) (full text)\"Unraveling Phosphorylation of the Splicing Factor SC35 by the Dual Signaling Kinase PKC in Human T-Cells\" Rao et al., Application Note (2016) (full text)\"Use of High-Density Histone Peptide Arrays for Parsing the Specificity of a Histone Modifying Enzyme Complex\" Wilczek et al., Application Note (2013) (full text)

Testimonial\"With JPT´s peptide microarray platform, high- throughput kinase profiling using small amounts of cell lysate becomes feasible, providing access to the discovery and monitoring of novel biomarkers in Parkinsons Disease.\" Jeremy Nichols, Parkinsonss Institute, Sunnyvale, USA

Phosphorylation Site-Detector PDK1

Technical Data:

Application	Phosphosite detectionSubstrate identification
Protein Name	PDK1
Organism	Homo sapiens (Human)
Indication / Topic	Kinase
Number of peptides	182

Further Information:

Amount	1 peptide microarray (each peptide is deposited 3x per subarray, 4 subarrays per slide) + dummy slide and spacers (needed for manual incubation)
Specifications	Peptide scan (13 mers with 10 aa overlap)
Documentation	CD with Protocol, Datasheet, gal-file and gal-viewer
Swiss-Prot ID	O15530
NCBI	NP_002604
Sequence	MARTTSQLYDAVPIQSSVVLCSCPSPSMVRTQTESSTPPGIPGGSRQGPAMDGTAAEPRPGAGSLQHAQPPPQPRKKRPEDFKFGKILGEGSFSTVVLARELATSREYAIKILEKRHIIKENKVPYVTRERDVMSRLDHPFFVKLYFTFQDDEKLYFGLSYAKNGELLKYIRKIGSFDETCTRFYTAEIVSALEYLHGKGIIHRDLKPENILLNEDMHIQITDFGTAKVLSPESKQARANSFVGTAQYVSPELLTEKSACKSSDLWALGCIIYQLVAGLPPFRAGNEYLIFQKIIKLEYDFPEKFFPKARDLVEKLLVLDATKRLGCEEMEGYGPLKAHPFFESVTWENLHQQTPPKLTAYLPAMSEDDEDCYGNYDNLLSQFGCMQVSSSSSSHSLSASDTGLPQRSGSNIEQYIHDLDSNSFELDLQFSEDEKRLLLEKQAGGNPWHQFVENNLILKMGPVDKRKGLFARRRQLLLTEGPHLYYVDPVNKVLKGEIPWSQELRPEAKNFKTFFVHTPNRTYYLMDPSGNAHKWCRKIQEVWRQRYQSHPDAAVQ
Protein Length (aa)	556