ActionsCite Favorites Display options Display options Format 1 Henan Key Laboratory of Cancer Epigenetic; Cancer Institute, The First Affiliated Hospital, and College of Clinical Medicine of Henan University of Science and Technology, Luoyang, China, 471003, Luoyang, 471003, China. 2 Department of Oncology, Anyang People s Hospital, Anyang, 471500, China. 3 Fudan-Zhangjiang Center for Clinical Genomics, Zuchongzhi Road 899, Shanghai, 201203, China. 4 Zhangjiang Center for Translational Medicine, Zuchongzhi Road 899, Shanghai, 201203, China. 5 Zhangjiang Center for Translational Medicine, Zuchongzhi Road 899, Shanghai, 201203, China. bpeng@biotecan.com. 6 Henan Key Laboratory of Cancer Epigenetic; Cancer Institute, The First Affiliated Hospital, and College of Clinical Medicine of Henan University of Science and Technology, Luoyang, China, 471003, Luoyang, 471003, China. samfeng137@hotmail.com. 7 Henan University of Science and Technology, Jing hua Road 24, Luoyang, 471500, China. samfeng137@hotmail.com. 1 Henan Key Laboratory of Cancer Epigenetic; Cancer Institute, The First Affiliated Hospital, and College of Clinical Medicine of Henan University of Science and Technology, Luoyang, China, 471003, Luoyang, 471003, China. 2 Department of Oncology, Anyang People s Hospital, Anyang, 471500, China. 3 Fudan-Zhangjiang Center for Clinical Genomics, Zuchongzhi Road 899, Shanghai, 201203, China. 4 Zhangjiang Center for Translational Medicine, Zuchongzhi Road 899, Shanghai, 201203, China. 5 Zhangjiang Center for Translational Medicine, Zuchongzhi Road 899, Shanghai, 201203, China. bpeng@biotecan.com. 6 Henan Key Laboratory of Cancer Epigenetic; Cancer Institute, The First Affiliated Hospital, and College of Clinical Medicine of Henan University of Science and Technology, Luoyang, China, 471003, Luoyang, 471003, China. samfeng137@hotmail.com. 7 Henan University of Science and Technology, Jing hua Road 24, Luoyang, 471500, China. samfeng137@hotmail.com. Gastric cardia adenocarcinoma (GCA), which occurs at the gastroesophageal boundary, is one of the most malignant types of cancer. Over the past 30 years, the incidence of GCA has increased by approximately sevenfold, which has a more substantial increase than that of many other malignancies. However, as previous studies mainly focus on non-cardia gastric cancer, until now, the mechanisms behind GCA remain largely unknown. MicroRNAs (miRNAs) have been shown to play pivotal roles in carcinogenesis. To gain insight into the molecular mechanisms regulated by miRNAs in GCA development, we investigated miRNA expression profiles using 81 pairs of primary GCAs and corresponding non-tumorigenic tissues. First, 21 pairs of samples were used for microarray analysis, and then another 60 pairs of samples were used for further analysis. Our results showed that 464 miRNAs (237 upregulated, 227 downregulated, false discovery rate FDR 0.05) were differently expressed between GCA and non-tumor tissues. Pearson test and pathway analysis revealed that these dysregulated miRNA correlated coding RNAs may have effects on several cancer-related pathways. Four miRNAs (miR-1244, miR-135b-5p, miR-3196, and miR-628-3p) were found to be associated with GCA differentiation. One miRNA, miR-196a-5p, was found to be associated with age of GCA onset. Further, survival analysis showed that the expression level of miR-135b-5p was associated with GCA survival. Taken together, our study first provided the genome-wide expression profiles of miRNA in GCA and will be good help for further functional studies. Differentiation; Gastric cardia adenocarcinoma; Microarray; miR135b-5p; microRNA. Li CY, Liang GY, Yao WZ, Sui J, Shen X, Zhang YQ, Peng H, Hong WW, Ye YC, Zhang ZY, Zhang WH, Yin LH, Pu YP. Li CY, et al. Clin Transl Oncol. 2017 Feb;19(2):162-172. doi: 10.1007/s12094-016-1516-y. Epub 2016 May 12. Clin Transl Oncol. 2017. PMID: 27173517 Mu YP, et al. 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Dose Response. 2020 Apr 23;18(2):1559325820920525. doi: 10.1177/1559325820920525. eCollection 2020 Apr-Jun. Dose Response. 2020. PMID: 32362797 Free PMC article. Biosci Rep. 2019 Dec 20;39(12):BSR20190845. doi: 10.1042/BSR20190845. Biosci Rep. 2019. PMID: 31803920 Free PMC article. Abdi E, et al. Cancer Med. 2019 Oct;8(13):6114-6126. doi: 10.1002/cam4.2497. Epub 2019 Aug 25. Cancer Med. 2019. PMID: 31448582 Free PMC article. Review.