Background:

CD39 contributes to immune regulation through the hydrolysis of ATP to AMP in the rate limiting step of extracellular adenosine production. CD39 is expressed on the surface of multiple cells of the immune system including T-cells, Natural killer cells, B-cells, dendritic cells and macrophages. In addition, high levels of CD39 expression have been associated with the tumor microenvironment (TME). Research aimed at reducing immunosuppression by CD39 activity has resulted in the study of a small molecule inhibitor, POM1, and multiple anti-CD39 antibodies selected for blocking enzymatic activityor decreasing CD39 expression.

Description:The CD39 Inhibitor Screening Assay Kit is designed to measure CD39 activity for screening and profiling applications. The CD39 assay kit comes in a convenient 96-well format, with purified recombinant CD39 enzyme, ATP, CD39 assay buffer, and Colorimetric detection reagent for 96 enzyme reactions. In addition, the kit includes the CD39 inhibitor POM-1 for use as an inhibitor control.UniProt P49961Synonym(s): Ubiquitin specific peptidase 7, USP 7, HAUSP, USP-7Supplied As: The CD39 assay kit comes in a convenient 96-well format, with purified recombinant CD39 enzyme, ATP, CD39 assay buffer, and Colorimetric detection reagent for 96 enzyme reactions.Format: Instructions for use: See assay kit data sheet for detailed protocol.Storage / Stability: Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.Application(s): Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.Reference(s): 1.Wang, T.-F., et al., J. Biol. Chem. 273 (38), 24814-21 (1998)Warning(s): Avoid freeze/thaw cycles.Scientific Category: ImmunotherapyProduct Type: Assay KitData shown is lot-specific. Contact us for specific information on other lots.