merck millipore,默克密理博,ABN78,Anti-NeuN (rabbit) Antibody产品名称：Anti-NeuN (rabbit) Antibody产品型号：ABN78ThisAnti-NeuN(rabbit)AntibodyisvalidatedforuseinWB,IH(P),ICforthedetectionofNeuN(rabbit).merck millipore,默克密理博,ABN78,Anti-NeuN (rabbit) AntibodyABN78 is a rabbit polyclonal version of the Anti-NeuN, clone A60 (MAB377), a highly characterized and cited mouse monoclonal antibody that specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found.Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.This Anti-NeuN (rabbit) Antibody is validated for use in WB, IH(P), IC for the detection of NeuN (rabbit).Immunohistochemistry Analysis: 1:1,000 dilution from a representative lot detected NeuN in normal mouse hippocampus and normal mouse midbrain tissues.Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected NeuN in rat E18 cortex/hippocampus primary cultures.Evaluated by Western Blot in mouse brain nuclear extract.Western Blot Analysis: 0.5 µg/mL of this antibody detected NeuN on 10 µg of mouse brain nuclear extract.Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.