The End-Repair mix converts DNA containing damaged or incompatible 5′- and/or 3′-protruding ends to 5′-phosphorylated, blunt-ended DNA. This high-concentration formulation of the End-Repair Mix is compatible with applications requiring >1 microgram of DNA to be prepared for blunt-end ligation. The conversion to blunt-ended DNA is accomplished by exploiting the 5′→3′ polymerase and 3′→5′ exonuclease activities of T4 DNA Polymerase (P7080). T4 Polynucleotide Kinase (Y9040) ensures that the ends of the blunt-ended DNA fragments are 5′-phosphorylated for subsequent ligation by T4 DNA Ligase (L6030-HC).Source of ProteinPurified from strains of E. coli that express the recombinant T4 DNA Polymerase, and T4 Polynucleotide Kinase genes, respectively.Supplied in100 mM KCl10 mM Tris-HCl0.1 mM EDTA1 mM DTT0.1% Triton X-10050% glycerolpH 7.4 @ 25°CSupplied WithB9140 (10X End-Repair Buffer)N2060 (1 mM dNTPs)10X End-Repair Buffer (B9140):1 M Tris-HCl500 mM NaCl100 mM MgCl250 mM DTT0.25% Triton-X 100pH 7.5 @ 25°C
Enzymatics--核酸25 mM 100µmol dNTP Solution Mix--N2050L,N2050F
核酸25mM100µmoldNTPSolutionMix
摘要:四种天然脱氧核苷酸的等摩尔混合溶液(25mM)
产品描述
Anequimolar(25mM)mixtureofthefournaturaldeoxynucleotides.
四种天然脱氧核苷酸的等摩尔混合溶液(25mM)
SuppliedAs:25mMeachdNTP(dATP,dCTP,dGTP,dTTP)atpH7.5
产品信息
PartNumber N2050L,N2050F
Concentration 25mM
UnitSize 100µmol,20µmol
产品特性
StorageTemperature -25to-15°C
Purity ≥99%
BasePurity ≥99.5%
Pyrophosphate ≤0.003pmol/pmolofnucleotide
EnzScript™(MMLV逆转录酶,RNaseH-)
摘要:基于RNA的DNA聚合酶,无核酸内切酶活性
产品描述:
EnzScript™(M-MLV逆转录酶RNaseHminus)属于RNA依赖的DNA聚合酶,该酶无RNaseH活性。EnzScript™被用于从polyAmRNA或总RNA中产生首链cDNA以用于下游的一些用途,如RT-PCR,cDNA克隆或RNA-Seq的文库构建。RNaseH结构域中的点突变能增加酶的热稳定性,相比于野生型M-MLV逆转录酶,它支持全长转录物种产生更高的cDNA产量