E.coli Endonuclease VIII functions as both an N-glycosylase (by excising oxidative base lesions) and an AP lyase (by subsequently cleaving the phosphodiester backbone), leaving terminal phosphates at the 5′ and 3′ ends. (1) Damaged bases removed by Endonuclease VIII include: urea, 5, 6- dihydroxythymine, thymine glycol, 5-hydroxy-5- methylhydanton, uracil glycol, 6-hydroxy-5, 6-dihydrothymine and methyltartronylurea (2,3).Source of ProteinAn E. coli strain which carries the cloned Endonuclease VIII gene.Supplied in10 mM Tris-HCl250 mM NaCl0.1 mM EDTA50% glycerolpH 8.0 @ 25°CSupplied With:B9080 10X Endonulease Vlll Buffer10X Endonuclease Vlll Buffer (B9080)100 mM Tris-HCl750 mM NaCl10 mM EDTApH 8.0 @ 25°CUnit DefinitionOne unit is defined as the amount of enzyme required to cleave 1 pmol of an oligonucleotide duplex containing a single AP site in 1 hour at 37°C.
Enzymatics--核酸25 mM 100µmol dNTP Solution Mix--N2050L,N2050F
核酸25mM100µmoldNTPSolutionMix
摘要：四种天然脱氧核苷酸的等摩尔混合溶液(25mM)
产品描述
Anequimolar(25mM)mixtureofthefournaturaldeoxynucleotides.
四种天然脱氧核苷酸的等摩尔混合溶液(25mM)
SuppliedAs:25mMeachdNTP(dATP,dCTP,dGTP,dTTP)atpH7.5
产品信息
PartNumber N2050L,N2050F
Concentration 25mM
UnitSize 100µmol,20µmol
产品特性
StorageTemperature -25to-15°C
Purity ≥99%
BasePurity ≥99.5%
Pyrophosphate ≤0.003pmol/pmolofnucleotide
EnzScript™(MMLV逆转录酶，RNaseH-)
摘要：基于RNA的DNA聚合酶，无核酸内切酶活性
产品描述：
EnzScript™(M-MLV逆转录酶RNaseHminus)属于RNA依赖的DNA聚合酶，该酶无RNaseH活性。EnzScript™被用于从polyAmRNA或总RNA中产生首链cDNA以用于下游的一些用途，如RT-PCR,cDNA克隆或RNA-Seq的文库构建。RNaseH结构域中的点突变能增加酶的热稳定性，相比于野生型M-MLV逆转录酶，它支持全长转录物种产生更高的cDNA产量
