RecA functions in DNA recombination and DNA repair (1,2,3). RecA binds to single stranded DNA, resulting in the polymerization of RecA into a nucleoprotein complex. This complex can align with complementary double stranded DNA, resulting in RecA catalysis of DNA strand exchange. RecA DNA binding is stimulated by ATP hydrolysis or non-hydrolyzable ATP analogs. The RecA-ATP-single stranded DNA complex also can function as a coprotease factor in the proteolytic cleavage of LexA, UmuD and certain bacteriophage proteins. RecA complexed with site-specific oligonucleotides have been used to target and specifically cleave large DNA fragments (4).Source of ProteinA recombinant E. coli strain overexpressing E. coli recA from a plasmid.Supplied in10 mM Tris-HCl1 mM DTT0.1 mM EDTA50% glycerolpH 7.5 @ 25°CSupplied With:B9260 (10X RecA Reaction Buffer)10X RecA Reaction Buffer (B9260):700 mM Tris-HCl100 mM MgCl250 mM DTTpH 7.6 @ 25°CUnit DefinitionSold by mass of pure protein determined atOD280 (A280 = 0.516 at 1 mg/mL, 1cm).
Enzymatics--核酸25 mM 100µmol dNTP Solution Mix--N2050L,N2050F
核酸25mM100µmoldNTPSolutionMix
摘要：四种天然脱氧核苷酸的等摩尔混合溶液(25mM)
产品描述
Anequimolar(25mM)mixtureofthefournaturaldeoxynucleotides.
四种天然脱氧核苷酸的等摩尔混合溶液(25mM)
SuppliedAs:25mMeachdNTP(dATP,dCTP,dGTP,dTTP)atpH7.5
产品信息
PartNumber N2050L,N2050F
Concentration 25mM
UnitSize 100µmol,20µmol
产品特性
StorageTemperature -25to-15°C
Purity ≥99%
BasePurity ≥99.5%
Pyrophosphate ≤0.003pmol/pmolofnucleotide
EnzScript™(MMLV逆转录酶，RNaseH-)
摘要：基于RNA的DNA聚合酶，无核酸内切酶活性
产品描述：
EnzScript™(M-MLV逆转录酶RNaseHminus)属于RNA依赖的DNA聚合酶，该酶无RNaseH活性。EnzScript™被用于从polyAmRNA或总RNA中产生首链cDNA以用于下游的一些用途，如RT-PCR,cDNA克隆或RNA-Seq的文库构建。RNaseH结构域中的点突变能增加酶的热稳定性，相比于野生型M-MLV逆转录酶，它支持全长转录物种产生更高的cDNA产量
