Bst X DNA Polymerase is a thermostable DNA polymerase from Geobacillus, homologous to the DNA polymerase from Bacillus stearothermophilus (Bst). Bst X lacks 5’→3′ and 3’→5′ exonuclease activity and exhibits a stronger strand-displacement activity than Manta 1.0 DNA Polymerase that may allow it to perform better in isothermal amplification technologies.Usage Notes:Optimal reaction temperature from 60-70°C.Heat inactivated at 80°C after 10 min incubation.High Tolerance to the non-ionic detergents Tween-20, Tween-80, TX-100, and NP-40 (up to 5% final concentration).Active in salt from 40 to 125 mM.Buffer Description10x Bst X Xcelerator Reaction Buffer, contains 2 mM MgSO4 at 1X concentration. Depending on the application, it may be necessary to adjust the MgSO4 from 2-10 mM final concentration.Source of ProteinA recombinant E.coli strain carrying the Bst X DNA Polymerase (exo-) polymerase gene.Unit Definition1 unit is defined as the amount of polymerase required to convert 10 nmol of dNTPs into acid insoluble material in 30 minutes at 65°CMolecular weight66,494 Daltons
Enzymatics--核酸25 mM 100µmol dNTP Solution Mix--N2050L,N2050F
核酸25mM100µmoldNTPSolutionMix
摘要:四种天然脱氧核苷酸的等摩尔混合溶液(25mM)
产品描述
Anequimolar(25mM)mixtureofthefournaturaldeoxynucleotides.
四种天然脱氧核苷酸的等摩尔混合溶液(25mM)
SuppliedAs:25mMeachdNTP(dATP,dCTP,dGTP,dTTP)atpH7.5
产品信息
PartNumber N2050L,N2050F
Concentration 25mM
UnitSize 100µmol,20µmol
产品特性
StorageTemperature -25to-15°C
Purity ≥99%
BasePurity ≥99.5%
Pyrophosphate ≤0.003pmol/pmolofnucleotide
EnzScript™(MMLV逆转录酶,RNaseH-)
摘要:基于RNA的DNA聚合酶,无核酸内切酶活性
产品描述:
EnzScript™(M-MLV逆转录酶RNaseHminus)属于RNA依赖的DNA聚合酶,该酶无RNaseH活性。EnzScript™被用于从polyAmRNA或总RNA中产生首链cDNA以用于下游的一些用途,如RT-PCR,cDNA克隆或RNA-Seq的文库构建。RNaseH结构域中的点突变能增加酶的热稳定性,相比于野生型M-MLV逆转录酶,它支持全长转录物种产生更高的cDNA产量