φ29 DNA Polymerase responsible for the replication of theBacillus Subtilis phage φ29 (1). The enzyme is a highly processive DNA polymerase (up to 70,000 base insertions per binding event) with a powerful strand displacement activity (2) and a 3′→ 5′ proofreading exonuclease function (3).Source of ProteinA recombinant E. coli strain carrying the φ29 DNA Polymerase gene from bacteriophage φ29.Supplied in 10 mM Tris-HCl100 mM KCl0.1 mM EDTA1 mM DTT0.5% Tween-200.5% NP-4050% glycerolpH 7.4 @ 25°CSupplied WithB7020 (10X φ29 DNA Polymerase Reaction Buffer)10X φ29 DNA Polymerase Buffer (B7020)500 mM Tris-HCl100 mM (NH4)2SO440 mM DTT100 mM MgCl2pH 7.5 @ 25°CUnit Definition1 unit is defined as the amount of polymerase required to convert 0.5 pmol of dTTP into acid insoluble materialin 10 minutes at 30°C.
Enzymatics--核酸25 mM 100µmol dNTP Solution Mix--N2050L,N2050F
核酸25mM100µmoldNTPSolutionMix
摘要：四种天然脱氧核苷酸的等摩尔混合溶液(25mM)
产品描述
Anequimolar(25mM)mixtureofthefournaturaldeoxynucleotides.
四种天然脱氧核苷酸的等摩尔混合溶液(25mM)
SuppliedAs:25mMeachdNTP(dATP,dCTP,dGTP,dTTP)atpH7.5
产品信息
PartNumber N2050L,N2050F
Concentration 25mM
UnitSize 100µmol,20µmol
产品特性
StorageTemperature -25to-15°C
Purity ≥99%
BasePurity ≥99.5%
Pyrophosphate ≤0.003pmol/pmolofnucleotide
EnzScript™(MMLV逆转录酶，RNaseH-)
摘要：基于RNA的DNA聚合酶，无核酸内切酶活性
产品描述：
EnzScript™(M-MLV逆转录酶RNaseHminus)属于RNA依赖的DNA聚合酶，该酶无RNaseH活性。EnzScript™被用于从polyAmRNA或总RNA中产生首链cDNA以用于下游的一些用途，如RT-PCR,cDNA克隆或RNA-Seq的文库构建。RNaseH结构域中的点突变能增加酶的热稳定性，相比于野生型M-MLV逆转录酶，它支持全长转录物种产生更高的cDNA产量
