RNA Methylation RNA methylation is a reversible post-translational modification to RNA that epigenetically impacts numerous biological processes. It occurs in different RNAs including tRNA, rRNA, mRNA, tmRNA, snRNA, snoRNA, miRNA, and viral RNA. Different catalytic strategies are employed for RNA methylation by a variety of RNA-methyltransferases.N6-methyladenosine (m6A) is the most common and abundant methylation modification in RNA molecules present in eukaryotes and accounts for more than 80% of all RNA methylation. It may be referred to as the \"fifth RNA base” and has broad roles in regulating embryonic development and cell fates. In particular, this RNA modification is thought to be important for mRNA regulation due to enrichment of m6A at 3’UTRs. It has also been linked to cancers including leukemia, breast cancer, and prostate cancer. N6-methyladenosine (m6A)5-methylcytosine (5-mC) is an epigenetic mark that also commonly occurs in various RNA molecules. Researchers are able to bisulfite convert RNA, perform RT-PCR amplification, cloning, and sequencing to gain information about RNA cytosine methylation states. By treating RNA with bisulfite, cytosine residues are deaminated to uracil while leaving 5-methylcytosine intact.Recent data strongly suggest that m6A and 5-mC RNA methylation affects the regulation of various biological processes such as RNA stability and mRNA translation, and that abnormal RNA methylation contributes to etiology of human diseases. Furthermore, loss of 5-mC in vault RNAs causes aberrant processing into Argonaute-associated small RNA fragments that can function as microRNAs. Researchers continue to investigate m6A and 5-mC in RNA to uncover the mysteries of RNA methylation.Purpose An ELISA-like format for absorbance-based quantitation of N6-methyladenosine in RNA An ELISA-like format for fluorescence-based quantitation of 5-mC RNA methylation For robust, chemical-based bisulfite treatment of RNA for analysis of methylated cytosinesKey Points Detect m6A RNA methylation in any species Colorimetric ELISA-like assay using an m6A antibody Detection limit as low as 10 pg of m6ALarge signal window, low background noiseDetect 5-mC RNA methylation in any speciesHigh specificity to 5-mC, with no cross-reactivity Input as low as 5 ng per reaction >90% of RNA loss preventedCompletely converts unmethylated RNA cytosine into uracil  Lead TimeExpress Only (2 Days or Less)Research KitsInput Type RNA(9) Nuclear Extracts(2) Purified Enzymes(2) Urine(1)Research Area RNA Methylation(12) Next Gen Sequencing(4)Target Application Amount Quantitation(6) Sample Modification(6) Library Construction(5) PCR Amplification(1)Vessel Format 96-Well Plate(7) Columns/Tubes(5)