Target: Human Host: Goat Antibody Format: Whole IgG Specificity: IgG (H+L) Minimal Cross Reactivity: Bovine, Horse, Mouse Serum Proteins Conjugate: 12 nm Colloidal GoldProduct Category: ImmunoGold Complexes Clonality: Polyclonal RRID: AB_2337748 Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule human IgG. It also reacts with the light chains of other human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, horse and mouse serum proteins, but it may cross-react with immunoglobulins from other species. Storage: Aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing. Prepare working dilution on day of use. Expiration date: one year from date of receipt. The expiration date may be extended if test results are acceptable for the intended use. Purity: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Buffer: 0.01M Sodium Borate - Sodium Phosphate, 0.15M NaCl, pH 8.5 Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) Preservative: 0.05% Sodium Azide Suggested Working Concentration or Dilution Range: Histo-/Cyto-Chemistry:- 1:20-1:40Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically. Colloidal gold reagents for transmission and scanning electron microscopy (EM Grade) are distinguished from other commercial preparations by careful separation of monomeric particles from small aggregates using ultracentrifugation in density gradients. The resulting monomeric colloidal gold-protein complexes are suspended in sterile-filtered buffer containing stabilizers and a preservative. All particle sizes in the EM Grade category may also be used for light microscopy and immunoblotting by those who prefer to use particles larger than 4 nm.This product is for IN VITRO RESEARCH USE ONLY. It is not a medical device and it is not intended for diagnostic or therapeutic purposes. You appear to be visiting this site from China . You may prefer to view this page on our site. You appear to be visiting this site from the UK. Orders for delivery to UK addresses should be placed directly with our UK distributor - Stratech Scientific Ltd.