Jackson公司715-625-150/AlexaFluor®680AffiniPureDonkey现货价格低

0.5mg

Target: MouseHost: DonkeyAntibodyFormat: WholeIgGSpecificity: IgG(H+L)

MinimalCrossReactivity: Bovine,Chicken,Goat,GuineaPig,SyrianHamster,Horse,Human,Rabbit,SheepSerumProteins

Conjugate: AlexaFluor®680ProductCategory: AlexaFluor®680andAlexaFluor®790forHighSensitivityWesternBlotsClonality: Polyclonal

WholeIgGantibodiesareisolatedasintactmoleculesfromantiserabyimmunoaffinitychromatography.TheyhaveanFcportionandtwoantigenbindingFabportionsjoinedtogetherbydisulfidebondsandthereforetheyaredivalent.Theaveragemolecularweightisreportedtobeabout160kDa.ThewholeIgGformofantibodiesissuitableforthemajorityofimmunodetectionproceduresandisthemostcosteffective.

Basedonimmunoelectrophoresisand/orELISA,theantibodyreactswithwholemoleculemouseIgG.Italsoreactswiththelightchainsofothermouseimmunoglobulins.Noantibodywasdetectedagainstnon-immunoglobulinserumproteins.TheantibodyhasbeentestedbyELISAand/orsolid-phaseadsorbedtoensureminimalcross-reactionwithbovine,chicken,goat,guineapig,syrianhamster,horse,human,rabbitandsheepserumproteins,butitmaycross-reactwithimmunoglobulinsfromotherspecies.

PhysicalState: Freeze-driedsolid Storage:

Storefreeze-driedpowderat2-8°C.Whenreadytouse,rehydratewithindicatedvolumeofd.waterandcentrifugeifnotclear.Productisstableforabout6weeksat2-8°Casanundilutedliquid.Prepareworkingdilutionfresheachday.Forextendedstorageafterrehydration,addanequalvolumeofglycerol(ACSgradeorbetter)forafinalconcentrationof50%,andstoreat-20°Casaliquid.Note:aftertheadditionofglycerol,theconcentrationofproteinandbuffersaltsisone-halfoftheoriginal.Alternatively,aliquotandfreezetheproductat-70°Corbelowintheabsenceofglycerol.Avoidrepeatedfreezingandthawing. Expirationdate: oneyearfromdateofrehydration.However,theexpirationdatemaybeextendediftheproductisstoredaccordingtotherecommendationandthetestresultsareacceptableforitsintendeduse.

Purity: Theantibodywaspurifiedfromantiserabyimmunoaffinitychromatographyusingantigenscoupledtoagarosebeads. Buffer: 0.01MSodiumPhosphate,0.25MNaCl,pH7.6 StABIlizer: 15mg/mlBovineSerumAlbumin(IgG-Free,Protease-Free) Preservative: 0.05%SodiumAzide SuggestedWorkingConcentrationorDilutionRange:

WesternBlot:-1:50,000-200,000Dilutionfactorsarepresentedintheformofarangebecausetheoptimaldilutionisafunctionofmanyfactors,suchasantigendensity,permeability,etc.Theactualdilutionusedmustbedeterminedempirically.

AlexaFluor®680

Amax: 684 Emax: 702nm

AlexaFluor®680andAlexaFluor®790conjugatesareusedforverysensitiveWesternblots,ELISAs,andmultiplexingarrays.AlexaFluor®680conjugatesareexcitedwithapeakaround684nmandfluorescewithapeakaround702nm.AlexaFluor®790conjugatesareexcitedwithapeakaround792nmandfluoresceatapeakaround803nm.TheyarethebestchoiceforhighlysensitivesingleordoublelabelingwithfluorescenceimagedinaLI-COROdyssey®imager.

ThisproductisforINVITRORESEARCHUSEONLY.Itisnotamedicaldeviceanditisnotintendedfordiagnosticortherapeuticpurposes.

ThisproductisprovidedunderanagreementbetweenLifeTechnologiesCorporationandJacksonImmunoResearchLaboratories,Inc,andthemanufacture,use,saleorimportofthisproductissoldpursuanttoalicensefromLifeTechnologiesCorporationforuseofitsfluorescentdyetechnology.Thesaleofthisproductisexpresslyconditionedonthebuyernotusingtheproductoritscomponents(1)inmanufacturing;(2)toprovideaservice,information,ordatatoanunaffiliatedthirdpartyforpayment;(3)fortherapeutic,diagnosticorprophylacticpurposes;(4)toresell,sellorotherwisetransferthisproductoritscomponentstoanythirdparty,orforanyothercommercialpurposes.Forinformationonpurchasingalicensetothisproductforpurposesotherthanresearch,contactLifeTechnologiesCorporation,5791VanAllenWay,Carlsbad,CA92008

美国JacksonImmunoResearchLaboratories,Inc.专业致力于亲合纯化的第二抗体和纯化的免疫球蛋白的生产和偶联。其产品深受广大研究人员的认可，销售范围遍布全球，领域包括植物，动物和生物医学研究。公司成立于1982年，公司创业者具有深厚的专业背景：细胞生物学，蛋白化学，微生物学，免疫学。公司自创立初，就将为其他研究人员提供最新最全的二抗作为公司的持续发展目标。