Jackson公司115-035-003/Peroxidase-AffiniPureGoatAnti-Mo现货价格低

2.0ml

Target: RabbitHost: GoatAntibodyFormat: WholeIgGSpecificity: IgG(H+L)Conjugate: HorserADIshPeroxidaseProductCategory: WholeIgGAffinity-PurifiedAntibodiesClonality: Polyclonal

WholeIgGantibodiesareisolatedasintactmoleculesfromantiserabyimmunoaffinitychromatography.TheyhaveanFcportionandtwoantigenbindingFabportionsjoinedtogetherbydisulfidebondsandthereforetheyaredivalent.Theaveragemolecularweightisreportedtobeabout160kDa.ThewholeIgGformofantibodiesissuitableforthemajorityofimmunodetectionproceduresandisthemostcosteffective.

Basedonimmunoelectrophoresisand/orELISA,theantibodyreactswithwholemoleculerabbitIgG.Italsoreactswiththelightchainsofotherrabbitimmunoglobulins.Noantibodywasdetectedagainstnon-immunoglobulinserumproteins.Theantibodymaycross-reactwithimmunoglobulinsfromotherspecies.

PhysicalState: Freeze-driedsolid Storage:

Storefreeze-driedpowderat2-8°C.Whenreadytouse,rehydratewithindicatedvolumeofd.waterandcentrifugeifnotclear.Productisstableforabout6weeksat2-8°Casanundilutedliquid.Prepareworkingdilutionfresheachday.Forextendedstorageafterrehydration,addanequalvolumeofglycerol(ACSgradeorbetter)forafinalconcentrationof50%,andstoreat-20°Casaliquid.Note:aftertheadditionofglycerol,theconcentrationofproteinandbuffersaltsisone-halfoftheoriginal.Alternatively,aliquotandfreezetheproductat-70°Corbelowintheabsenceofglycerol.Avoidrepeatedfreezingandthawing. Expirationdate: oneyearfromdateofrehydration.However,theexpirationdatemaybeextendediftheproductisstoredaccordingtotherecommendationandthetestresultsareacceptableforitsintendeduse.

Purity: Theantibodywaspurifiedfromantiserabyimmunoaffinitychromatographyusingantigenscoupledtoagarosebeads. Buffer: 0.01MSodiumPhosphate,0.25MNaCl,pH7.6 StABIlizer: 15mg/mlBovineSerumAlbumin(IgG-Free,Protease-Free) Preservative: None(Warning:Useofsodiumazideasapreservativewillsubstantiallyinhibittheenzymeactivityofhorseradishperoxidase.) SuggestedWorkingConcentrationorDilutionRange:

1:500-1:5,000forimmunohisto/cytochemistry1:5,000-1:100,000forELISAandWesternblottingwithchromogenicsubstrates1:10,000-1:200,000forWesternblottingwithECLsubstratesDilutionfactorsarepresentedintheformofarangebecausetheoptimaldilutionisafunctionofmanyfactors,suchasantigendensity,permeability,etc.Theactualdilutionusedmustbedeterminedempirically.

HorseradishPeroxidase

Horseradishperoxidase(HRP)conjugatesarepreparedbyamodifiedNakaneandKawaoiprocedure(J.Histochem.Cytochem.1974. 22,1084).Peroxidaseconjugatesarecommonlyusedforimmunohistochemistry,Westernblotting,andELISA.Affinity-purifiedanti-horseradishperoxidaseandconjugatesareavailablefordetectionofhorseradishperoxidaseantigenorforsignalamplificationofHRP-containingreagents.Forimmunostainingofmammaliancells,anadvantageofusinganti-horseradishperoxidaseisreducedbackground,sincetheantibodydoesnotrecognizetheendogenousperoxidase-likeenzymesfoundinthosecells.

美国JacksonImmunoResearchLaboratories,Inc.专业致力于亲合纯化的第二抗体和纯化的免疫球蛋白的生产和偶联。其产品深受广大研究人员的认可，销售范围遍布全球，领域包括植物，动物和生物医学研究。公司成立于1982年，公司创业者具有深厚的专业背景：细胞生物学，蛋白化学，微生物学，免疫学。公司自创立初，就将为其他研究人员提供最新最全的二抗作为公司的持续发展目标。