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用于高分辨率免疫染色的新型 VHH 片段抗体

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为您的应用选择合适的亲和纯化二抗

关于荧光团、生物素-SP（长间隔基）和与亲和纯化抗体和其他酶偶联的酶的技术信息蛋白质

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关闭多克隆 AffiniPure-VHH™ 二抗放大细节

用于高分辨率免疫染色的新型 VHH 片段抗体

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关闭公告 增加通量仍然是对基于神经元的区室化培养平台的巨大需求。目前使用 PDMS 的方法不足以创建更高密度的平台，因为收缩导致的配准问题几乎不可能实现自动成像。 Xona Microfluidics 通过开发一种称为 X4 芯片的新型注塑 XonaChip 克服了这一限制。该芯片含有始终位于相同位置和大小的多个样本单元是自动化成像和药物发现工作的巨大进步。此外，这些芯片（标准显微镜载玻片的大小）可以组合在板架中以产生十六 (16) 个样品单元。查看这篇文章以了解更多信息。公告 增加通量仍然是对基于神经元的区室化培养平台的巨大需求。目前使用 PDMS 的方法不足以创建更高密度的平台，因为收缩导致的配准问题几乎不可能实现自动成像。 Xona Microfluidics 通过开发一种称为 X4 芯片的新型注塑 XonaChip 克服了这一限制。该芯片含有始终位于相同位置和大小的多个样本单元是自动化成像和药物发现工作的巨大进步。此外，这些芯片（标准显微镜载玻片的大小）可以组合在板架中以产生十六 (16) 个样品单元。查看这篇文章以了解更多信息。公告 增加通量仍然是对基于神经元的区室化培养平台的巨大需求。目前使用 PDMS 的方法不足以创建更高密度的平台，因为收缩导致的配准问题几乎不可能实现自动成像。 Xona Microfluidics 通过开发一种称为 X4 芯片的新型注塑 XonaChip 克服了这一限制。该芯片含有始终位于相同位置和大小的多个样本单元是自动化成像和药物发现工作的巨大进步。此外，这些芯片（标准显微镜载玻片的大小）可以组合在板架中以产生十六 (16) 个样品单元。查看这篇文章以了解更多信息。

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with human lactoferrin in human milk.No antibody was detected against other human milk or serum proteins.The antibody may cross-react with lactoferrin from other species.Physical State: Freeze-dried solid Storage:

Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Note: after the addition of glycerol, the concentration of protein and buffer salts is one-half of the original. Alternatively, aliquot and freeze the product at -70°C or below in the absence of glycerol. Avoid repeated freezing and thawing.Expiration date: one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.

Purity:The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 Stabilizer:15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) Preservative:None(Warning:Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.) Suggested Working Concentration or Dilution Range:ELISA:- 1:5,000-1:100,000Western Blot:- 1:5,000-1:100,000 (non-ECL) / 1:10,000-1:200,000 (ECL)Histo-/Cyto-Chemistry:- 1:500-1:5,000Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

Horseradish Peroxidase

Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.