Accept cookiesWe use cookies to understand how you use our site and to improve the overall user experience. This includes personalizing content and advertising. To learn more and manage cookies, please refer to our Cookie Statement.Are you doing COVID-19 related research? Our latest RUO kit, the Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit, enables high throughput workflows for real-time detection of SARS-CoV-2 nucleic acid using hydrolysis probes. For simple, visual assay results, the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit includes a color-changing pH indicator for detection of SARS-CoV-2 nucleic acid amplificationLearn about our tools that are helping researchers develop diagnostics and vaccines for the SARS-CoV-2 virus.Time for ChangeMonarch Nucleic Acid Purification Kits are optimized for maximum performance and minimal environmental impact. Kits are available for total RNA purification, plasmid miniprep, gel extraction, and DNA RNA cleanup. For maximum convenience and value, columns and buffers are also available separately. Learn more and request a sample!Single-stranded (ssDNA or RNA) specific endonucleaseNuclease P1 is ideal for:Conversion of single-stranded DNA or RNA to 5\'-mononucleotides Analysis of the base composition of nucleic acidsStudies of the potential damage and modification in DNARequest a copy of our Choosing the Right Exonuclease poster.To find out how to order this product from your current location, click the button below: International Customer Order Support Nuclease P1 is a zinc-dependent single-strand specific nuclease which hydrolyzes phosphodiester bonds in RNA and ssDNA with no base specificity. Nuclease P1 also exhibits 3 -phosphomonoesterase activity (1). Although a single-strand specific nuclease (ssDNA and RNA-specific), Nuclease P1 does display some activity toward dsDNA in Nuclease P1 Reaction Buffer. If preferentially degrading single-stranded nucleic acids (ssDNA or RNA) in the presence of double stranded DNA (dsDNA), we recommend using Nuclease P1 in 1X NEBuffer 1.1 to limit activity on dsDNA while maintaining single-strand nuclease activity.Product SourceIsolated from Penicillium citrinum. Reagents SuppliedThe following reagents are supplied with this product:Unit DefinitionOne unit is defined as the amount of enzyme required to liberate 1.0 g of acid soluble nucleotides from Torula Yeast total RNA per min at 37 C in 1X Nuclease P1 Reaction Buffer.Reaction Conditions1X Nuclease P1 Reaction Buffer Incubate at 37 C 1X Nuclease P1 Reaction Buffer50 mM sodium acetate (pH 5.5 @ 25 C)Storage Buffer25 mM Tris-HCl 50 mM NaCl 1 mM ZnCl2 50% Glycerol pH 7.2 @ 25 C Heat Inactivation75 C for 10 min Substrate specificity for Nuclease P1 is as follows: 3 AMP RNA ssDNA dsDNA (1). The rate of hydrolysis of 2 -AMP is 3,000-fold less than that of 3 -AMP (1). Although a single-strand specific nuclease (ssDNA and RNA-specific), Nuclease P1 does display some activity toward dsDNA in Nuclease P1 Reaction Buffer. If preferentially degrading single-stranded nucleic acids (ssDNA or RNA) in the presence of double stranded DNA (dsDNA), we recommend using Nuclease P1 in 1X NEBuffer 1.1 to limit activity on dsDNA while maintaining single-strand nuclease activity. Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.SpecificationsThe Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version] M0660S_v1 This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.For more information about commercial rights, please contact NEB\'s Global Business Development team at gbd@neb.com.This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.New England Biolabs (NEB) is committed to practicing ethical science we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.Ineligible item added to cartBased on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly If you have any questions, please contact Customer Service at freezers@neb.com or 1-800-632-5227 x 8.If you don t see your country above, please visit ourinternational siteSession ExpiredYou have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.Sign InYour profile has been mapped to an Institution, please sign back for your profile updates to be completed.Sign InTo save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.