Description:

   Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity (1,2,3) and a 5´ flap endonuclease activity (4,5). Quick-Load® Taq 2X Master Mix is an optimized ready-to-use solution containing Taq DNA Polymerase, dNTPs, MgCl₂, KCl, tracking dyes and stabilizers. The presence of two commonly used tracking dyes for DNA gels, Orange G and Xylene Cyanol FF, gives the master mix a green color, allowing direct loading of the PCR product onto agarose gels. On a 1% agarose gel in 1X TBE, Xylene Cyanol FF migrates at approximately 4 kb and Orange G migrates at approximately 50 bp. The amount of tracking dyes included does not mask co-migrating DNA bands. Quick-Load Taq 2X Master Mix is ideally suited to routine PCR applications from templates including pure DNA solutions, bacterial colonies, and cDNA products. It can amplify up to 4 kb from complex genomic DNA or up to 5 kb from lambda DNA.

Highlights

• Robust and reliable reactions
• Tolerates a wide range of templates
• Exceptional value in terms of cost per unit

Product Source

An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1.

Reagents Supplied

The following reagents are supplied with this product:

	Store at (°C)	Concentration
Magnesium Chloride (MgCl2) Solution	-20	25 mM

Notes:

Keep at -20°C for long term storage. Quick-Load Taq 2X Master Mix is stable for fifteen freeze-thaw cycles when stored at -20°C.Quick-Load Taq 2X Master Mix is also stable for one week at 4°C, so for daily use, an aliquot may be kept at 4°C.Quick-Load Taq 2X Master Mix should be used at a 1X concentration with DNA template and primers in a total reaction volume of 25 or 50 μl.