Description:

RNase Inhibitor, Human Placenta is a recombinant human placental protein which specifically inhibits ribonucleases (RNases) A, B and C (1). It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus. In addition, no inhibition of polymerase activity is observed when RNase Inhibitor, Human Placenta is used with Taq DNA Polymerase, AMV or M-MuLV Reverse Transcriptases, or Phage RNA Polymerases (SP6, T7, or T3).The 50 kDa protein inhibits RNases by binding noncovalently in a 1:1 ratio with an association constant greater than 10¹⁴ (2).

Highlights

• Isolated from a recombinant source
• Inhibits common eukaryotic RNases
• Compatible with Taq Polymerase, AMV or M-MuLV Reverse Transcriptases
• Active over a broad pH range (pH 5-8)

Product Source

An E. coli strain that carries the Ribonuclease Inhibitor gene from human placenta.

Notes:

Since ribonucleases typically retain activity under denaturing conditions, care must be taken to avoid denaturing RNase Inhibitor molecules which have complexed with a ribonuclease. To prevent the release of active ribonuclease, temperatures greater than 50°C and high concentrations of urea or other denaturing agents should be avoided.The recommended concentration of RNase Inhibitor in a reaction is 1 unit/μl. During assembly of a reaction, RNase Inhibitor should be added before other components that are possible sources of RNase contamination (i.e. enzymes, plasmid from a mini prep.)