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登入 |鼻,NEB/PvuII/R0151S/5,000 units

Description:

PvuII has a High Fidelity version PvuII-HF® (NEB #R3151).

High Fidelity (HF®) Restriction Enzymes have 100% activity in CutSmart™ Buffer; single-buffer simplicity means more straightforward and streamlined sample processing.  HF enzymes also exhibit dramatically reduced star activity.  HF enzymes are all Time-Saver™ qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA.  Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.  

PvuII crystals (Ira Schildkraut and Joseph Bonventre, New England Biolabs)\"PvuII

Product Source

An E.coli strain that carries the PvuII gene from Proteus vulgaris (ATCC 13315).

Reagents Supplied

The following reagents are supplied with this product:

Store at (°C)Concentration
NEBuffer™ 3.1-2010X

Notes:

May exhibit star activity in CutSmart Buffer.PvuII does not cut CAGm4CTG, while cutting at CAGm5CTG occurs at a significantly reduced rate.For enzymes that cannot be heat-inactivated, we recommend using a column for cleanup (such as the Monarch® PCR & DNA Cleanup Kit), or running the reaction on an agarose gel and then extracting the DNA (we recommend Monarch Gel Extraction Kit), or performing a phenol/chloroform extraction.