Description:
NEB extensively performs quality controls on all standard and high-fidelity(HF®) restriction enzymes. Examples of nuclease contamination studies for someof our HF restriction enzymes are shown below.Restriction Enzyme Competitor Study: Nuclease Contamination
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EcoRI, NotI, and BamHI from multiple suppliers were tested in reactionscontaining a fluorescent labeled single stranded, double stranded blunt,3’overhang or 5’ overhang containing oligonucleotides. The percent degradationis determined by capillary electrophoresis and peak analysis. The resolution isat the single nucleotide level. Product Source
An E.coli strain that carries the cloned and modified BsrGI gene from Bacillus stearothermophilus GR75 (Z. Chen)Reagents Supplied
The following reagents are supplied with this product:
| Store at (°C) | Concentration | |
| CutSmart® Buffer | -20 | 10X |
