Description:

Bsu DNA Polymerase I, Large Fragment retains the 5´→ 3´ polymerase activity of the Bacillus subtilis DNA polymerase I (1), but lacks the 5´→ 3´ exonuclease domain. This large fragment naturally lacks 3´→ 5´ exonuclease activity.

Product Source

An E. coli strain that contains a genetic fusion of the Bacillus subtilis DNA polymerase I gene (starting from codon 297 thus lacking the 5´→ 3´ exonuclease domain), and the gene coding for maltose binding protein (MBP). The fusion protein is purified to near homogeneity and the MBP portion is cleaved off in vitro. The remaining DNA polymerase is purified free of MBP.

Reagents Supplied

The following reagents are supplied with this product:

	Store at (°C)	Concentration
NEBuffer™ 2	-20	10X

Notes:

Bsu DNA Polymerase, Large Fragment is not suitable for generating blunt ends because it lacks the 3´→ 5´ exonuclease necessary to remove non-templated 3´ additions.Bsu DNA Polymerase, Large Fragment retains 50% activity at 25°C and is twice as active as Klenow Fragment (3´→ 5´ exo–) at this temperature.