Description:

EagI has a High Fidelity version EagI-HF® (NEB #R3505).High Fidelity (HF®) Restriction Enzymes have 100% activity in CutSmart™ Buffer; single-buffer simplicity means more straightforward and streamlined sample processing.HF enzymes also exhibit dramatically reduced star activity.HF enzymes are all Time-Saver™ qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA.Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.

Product Source

An E. coli strain that carries the EagI gene from Enterobacter agglomerans (R. Morgan).

Reagents Supplied

The following reagents are supplied with this product:

	Store at (°C)	Concentration
NEBuffer™ 3.1	-20	10X

Notes:

EagI is an isoschizomer of XmaIII.For full EagI activity, the pH of the reaction mix must be between (7.9 and 9.0 @ 25°C).This enzyme has shown to have lower activity on some supercoiledplasmids, with more than 1 unit required to digest 1 μg plasmid DNA. Forcomplete digestion of 1 μg of plasmid DNA please follow our recommendeddigestion protocol.