Description:
Product Source
An E. coli strain that carries the Fnu4HI gene from Fusobacterium nucleatum 4H (M. Smith).
Reagents Supplied
The following reagents are supplied with this product:
| Store at (°C) | Concentration |
| CutSmart® Buffer | -20 | 10X |
Notes:
Fnu4HI produces DNA fragments that have a single-base 5´ extension which are more difficult to ligate than blunt-ended fragments.For enzymes that cannot be heat-inactivated, we recommend using a column for cleanup (such as the Monarch® PCR & DNA Cleanup Kit), or running the reaction on an agarose gel and then extracting the DNA (we recommend Monarch Gel Extraction Kit), or performing a phenol/chloroform extraction.
