Description:

E. coli RecA is necessary for genetic recombination, reactions involving DNA repair and UV-induced mutagenesis. RecA promotes the autodigestion of the lexA repressor, umuD protein and lambda repressor. Cleavage of LexA derepresses more than 20 genes (1). In vitro studies indicate that in the presence of ATP, RecA promotes the strand exchange of single-strand DNA fragments with homologous duplex DNA. The reaction has three distinct steps: (i) RecA polymerizes on the single-strand DNA, (ii) the nucleo­protein filament binds the duplex DNA and searches for a homologous region, (iii) the strands are exchanged (2).

Reagents Supplied

The following reagents are supplied with this product:

	Store at (°C)	Concentration
Rec A Reaction Buffer		10X

Notes:

ATPγS which is required for triple helix formation is not supplied.Heat Inactivation: 65°C for 20 min