Description:
BglI crystals (Ira Schildkraut and John Greci,
New England Biolabs)
![\"BglI](\"https://www.ebiomall.com/images/neb/201709/R0143WG.gif\")
Product Source
An E. coli strain that carries the BglI gene from Bacillus globigii (ATCC 49760).
Reagents Supplied
The following reagents are supplied with this product:
| Store at (°C) | Concentration |
| NEBuffer™ 3.1 | -20 | 10X |
Notes:
The various sticky ends produced by BglI cleavage can be used to reconstitute plasmid and phage genomes and to exchange wild-type and mutant DNA fragments (Burger, K. J. and Schinzel, R. (1983) Mol. Gen. 189, 269-274).This enzyme has shown to have lower activity on some supercoiledplasmids, with more than 1 unit required to digest 1 μg plasmid DNA. Forcomplete digestion of 1 μg of plasmid DNA please follow our recommendeddigestion protocol.
