Description:

Product Source

An E. coli strain that carries the HphI gene from Haemophilus parahaemolyticus (ATCC 49700).

Reagents Supplied

The following reagents are supplied with this product:

	Store at (°C)	Concentration
CutSmart® Buffer	-20	10X

Notes:

It has been suggested that HphI may cleave at N9/N8 depending on the sequence between the recognition and cleavage sites (Kang, C. and Wu, C.-W. (1987) Nucl. Acids Res. 15, 2279-2294; Cho, S.-H. and Kang, C. (1990) Mol. Cells 1, 81?86.). Incubation of > 12 units for over 4 hours on ΦX174 DNA results in additional cleavage products. This has not yet been shown to occur on other DNAs. Low pH and high glycerol concentration enhance this activity.This enzyme is blocked by dam methylation. More information can be found at Dam-Dcm and CpG Methylation.This enzyme is blocked by dcm methylation. More information can be found at Dam-Dcm and CpG Methylation.Star activity may result from extended digestion, high enzyme concentration or a glycerol concentration of >5%