Description:
Product Source
An E. coli strain that carries the ApaLI gene from Acetobacter pasteurianus (ATCC 12875).
Reagents Supplied
The following reagents are supplied with this product:
| Store at (°C) | Concentration |
| CutSmart® Buffer | -20 | 10X |
Notes:
Cleavage of mammalian genomic DNA is blocked by overlapping CpG methylation.ApaLI does not cut M13 DNA. Reports of a single site in M13 DNA have been attributed to a sequencing error. The corrected sequence is GTGCTC, which can be cleaved by either BsiHKAI or Bsp1286I.For enzymes that cannot be heat-inactivated, we recommend using a column for cleanup (such as the Monarch® PCR & DNA Cleanup Kit), or running the reaction on an agarose gel and then extracting the DNA (we recommend Monarch Gel Extraction Kit), or performing a phenol/chloroform extraction.
