通过更新搜索字段、选择过滤器类型和应用其他过滤器来优化搜索。
您的搜索返回了 1328 个结果。显示：1050100250All«1…234567891011…133» 搜索结果TitleTypeAgencourt® Ampure® Bead (Beckman Coulter) Preparation (E6080)ProtocolAgencourt® Ampure® Beads (Beckman Coulter) Preparation - NEBNext Quick DNA Sample Prep Master Mix Set for 454 End Repair and dA-Tailing (E6090)ProtocolAmplification Protocol for PicoPLEX WGA Kit协议使用 Phusion 高保真 PCR 扩增控制模板试剂盒使用 PURExpress 分析合成蛋白质的方案 (E3313)使用 PURExpress 分析合成蛋白质的方案 (E6800)使用 PURExpress 分析合成蛋白质的方案 (E6840)使用 PURExpress 分析合成蛋白质的方案 (E6850)协议附录 A 用于 NEBNext Ultra II RNA 第一链合成的方案附录 A模块（E7771）协议HomeTools & ResourcesSearchSearch通过更新搜索字段、选择过滤器类型和应用其他过滤器来优化您的搜索。
您的搜索返回了 1328 个结果。显示：1050100250All«12345678910…133» 搜索结果TitleTypeAdaptor Ligation of End Repaired DNA (E6260) dA 尾 DNA 的协议适配器连接 (E6040)cDNA 文库的协议适配器或向量连接 (E6100)cDNA 文库的协议适配器或向量连接 (E6114)协议亲和纯化和柱上切割 (E6901)协议亲和纯化和柱上切割 (NEB) #S6651)Agarose DigestionProtocolAgencourt AMPure XP Bead Clean-up and Size Selection of End Repaired DNA (E6260)ProtocolAgencourt Ampure Beads (Beckman Coulter) Preparation (E6115)ProtocolAgencourt Ampure Beads (Beckman Coulter) Preparation (E6116) 协议HomeTools & ResourcesSearchSearch通过更新搜索字段、选择过滤器类型和应用其他过滤器来优化您的搜索。
您的搜索返回了 1328 个结果。显示：1050100250All«12345678910…133» 搜索结果 TitleType6C。使用 AMPure XP Beads NEBNext Small RNA Library Prep Set for Illumina（E7300、E7580、E7560、E7330）进行质量控制检查和大小选择使用灰色牛肝菌凝集素 (BGL) 的表位定向聚糖富集 (EDGE) 分析方案 (NEB #P0867)ProtocolA 典型的核酸外切酶 V 反应 (M0345)ProtocolA 典型的加尾反应 (M0337)ProtocolA-Tailing（单核苷酸）与末端转移酶ProtocolA-Tailing with Klenow片段 (3\'-->5\' exo-)ProtocolA-Tailing with Taq PolymeraseProtocolHomeTools & ResourcesSearchSearch通过更新搜索字段、选择过滤器类型和应用其他过滤器来优化您的搜索。
您的搜索返回了 1328 个结果。显示：1050100250All«12345678910…133» 搜索结果TitleType5 分钟转换协议 (C3032)Protocol5 分钟转化方案 (C3037)Protocol5 Minute Transformation Protocol (C3040)Protocol5 Minute Transformation Protocol for Competent E. coliProtocol5 Minute Transformation Protocol using NEB 10-beta Competent E. coli (C3019H/C3019I)Protocol52-Fragment Golden Gate Assembly using BsaI-HF®v2 (NEB #E1601)Protocol5\' RACE 协议使用模板转换 RT 酶混合物 (NEB #M0466)Protocol5\'-在使用 Phusion 定点诱变试剂盒 (F-541)Protocol6A 之前对寡核苷酸进行磷酸化。使用适用于 Illumina（E7300、E7580、E7560、E7330）协议 6B 的 6% 聚丙烯酰胺凝胶 NEBNext 小 RNA 文库准备套装进行质量控制检查和大小选择。质量控制检查和尺寸选择使用 Pippin Prep NEBNext Small RNA Library Prep Set for Illumina (E7300, E7580, E7560, E7330)Protocol 进行选择HomeTools & ResourcesSearchSearch通过更新搜索字段、选择过滤器类型和应用其他过滤器来优化您的搜索。
您的搜索返回了 1328 个结果。显示：1050100250All«12345678910…133» 搜索结果TitleType5 分钟转换协议 (C3010)Protocol5 分钟Protocol5分钟转换协议(C3013)Protocol5分钟转换协议(C3016)Protocol5分钟转换协议(C3022)Protocol5分钟转换协议(C3025)Protocol5分钟转换协议(C3026)Protocol5分钟转换协议(C3027)Protocol5分钟转换协议(C3028)Protocol5分钟转换协议协议(C3029)协议5分钟转换协议(C3030)协议HomeTools & ResourcesSearchSearch通过更新搜索字段、选择过滤器类型和应用其他过滤器来优化您的搜索。
您的搜索返回了 1328 个结果。显示：1050100250All«12345678910…133» 搜索结果TitleType5 分钟转换协议 (C2527)Protocol5 分钟Protocol5分钟变换协议(C2529)Protocol5分钟变换协议(C2530)Protocol5分钟变换协议(C2566)Protocol5分钟变换协议(C2984)Protocol5分钟变换协议(C2987H/C2987I)Protocol5分钟变换协议(C2988)Protocol5分钟变换协议(C2992)协议5分钟变换协议(C3009)协议主页常见问题解答 ACP 标签底物对固定稳定吗？常见问题解答：ACP 标签底物对固定稳定吗？ACP 标签底物的荧光来源于有机荧光团。因此，标记的 ACP 标签融合蛋白对固定非常稳定，并且与某些 GFP 光谱变体相比不会丢失信号强度。
HomeFAQsAre 4 standards enough for a qPCR quantitation?FAQ:Are 4 standards enough for a qPCR quantitation?是的。尽管我们建议使用 6 个标准品 (100 pM – 0.001pM)，但从 4 个标准品 (10 pM – 0.01pM) 生成的标准曲线足以可靠地测量文库浓度。即使一个标准品由于设置问题而偏离曲线，其他 3 个标准品也可以放心使用。随试剂盒提供的标准浓度范围将涵盖所有使用标准方法和试剂盒制备的文库，这些文库遵循试剂盒稀释文库指南。此资源的链接相关产品：NEBNext® 用于 Illumina® 的文库定量试剂盒、E7642 NEBNext 文库定量 DNA 标准品Accept cookiesWe use cookies to understand how you use our site and to improve the overall user experience. This includes personalizing content and advertising. To learn more and manage cookies, please refer to our Cookie Statement.Are you doing COVID-19 related research? Our latest RUO kit, the Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit, enables high throughput workflows for real-time detection of SARS-CoV-2 nucleic acid using hydrolysis probes. For simple, visual assay results, the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit includes a color-changing pH indicator for detection of SARS-CoV-2 nucleic acid amplificationLearn about our tools that are helping researchers develop diagnostics and vaccines for the SARS-CoV-2 virus.Product Price ListDownload a PDF containing pricing for our full product list.Free ShippingSave time and money by placing an order with NEB. Take advantage of free shipping for any order totaling over $350. Place your order before 7:30pm EST for overnight delivery.For shipping questions click here.This product has been discontinued. Quick CIPis a suitable alternative.Alkaline Phosphatase, Calf Intestinal (CIP) nonspecifically catalyzes the dephosphorylation of 5 and 3 ends of DNA and RNA phosphomonoesters.Faster reaction setup (no supplemental additives like zinc required)Flexible reaction conditions (active in any restriction enzyme buffer, no clean-up required)Less enzyme required (high specific activity), resulting in a lower cost per reactionNo need for multiple phosphatases (CIP removes 5 - and 3 - phosphates from DNA, RNA and dNTPs)Active on unincorporated dNTPs in PCR products - improves DNA sequencing and SNP analysisStorage Temperature: -20 Chydrolyses ribo-, as well as deoxyribonucleosidetriphosphates (NTPs and dNTPs). CIP is useful inmany molecular biology applications such as theremoval of phosphorylated ends of DNA and RNAfor subsequent use in cloning or end-labeling ofprobes. In cloning, dephosphorylation preventsreligation of linearized plasmid DNA. The enzymeacts on 5 protruding, 5 recessed and blunt ends.CIP may also be used to degrade unincorporateddNTPs in PCR reactions to prepare templates forDNA sequencing or SNP analysis.Product SourceCalf intestinal mucosa Product Categories:Discontinued ProductsFeatures Faster reaction setup (no supplemental additives like zinc required) Flexible reaction conditions (active in any restriction enzyme buffer, no clean-up required) Less enzyme required (high specific activity), resulting in a lower cost per reaction No need for multiple phosphatases (CIP removes 5 - and 3 - phosphates from DNA, RNA and dNTPs) Active on unincorporated dNTPs in PCR products - improves DNA sequencing and SNP analysis Application Features Dephosphorylation 5 and 3 ends of DNA and RNA. Dephosphorylation of cloning vector DNA to prevent recircularization during ligation. Dephosphorylation of DNA prior to end-labeling using T4 Polynucleotide Kinase. Treatment of dNTPs in PCR reactions prior to sequencing or SNP analysis.Unit DefinitionOne unit is defined as the amount of enzyme that hydrolyzes 1 mol of p-Nitrophenyl Phosphate, PNPP (NEB #P0757) in a total reaction volume of 1 ml in 1 minute at 37 C.Reaction Conditions1X CutSmart Buffer Incubate at 37 C 1X CutSmart Buffer50 mM Potassium acetate 20 mM Tris-acetate 10 mM Magnesium acetate 100 µg/ml BSA (pH 7.9 @ 25 C)Storage Buffer10 mM Tris-HCl 50 mM KCl 1 mM MgCl2 50% Glycerol 0.1 mM ZnCl2 pH 8.2 @ 25 C Heat InactivationMolecular WeightTheoretical: 69 kDa Unit Assay Conditions1 M diethanolamine- HCl (pH 9.8), 0.5 mM MgCl2, 50 mM p-nitrophenyl Phosphate and enzyme. These conditions are only used for quantitating enzyme activity. CIP, as are most alkaline phosphatases, isa Zn2+ and Mg2+-dependent enzyme. Ourformulation of its storage buffer provides Zn2+and Mg2+, which does not require supplementalzinc or other additives in reactions with CIP. CIP is also active in 1X NEBuffers 1.1, 2.1,3.1, as well as NEBuffers 1, 2, 3, 4 and uniqueNEBuffer for EcoRI. CIP activity is enhanced in the presence ofmonovalent salts. CIP is inhibited by metal chelators (e.g. EDTA),inorganic phosphate and phosphate analogs. CIP activity is decreased in the presence of reducing agents (DTT, -ME). Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, (2nd ed.). (p 5.72), Cold Spring Harbor Laboratory Press. Mossner, E., Boll, M. and Pfleiderer, G. (1980). Hoppe Seylers Z. Physiol Chem. 361, Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.SpecificationsThe Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version] M0290S_L_v2 This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.For more information about commercial rights, please contact NEB\'s Global Business Development team at gbd@neb.com.This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.New England Biolabs (NEB) is committed to practicing ethical science we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.Ineligible item added to cartBased on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly If you have any questions, please contact Customer Service at freezers@neb.com or 1-800-632-5227 x 8.If you don t see your country above, please visit ourinternational siteSession ExpiredYou have been idle for more than 20 minutes, for your security you have been logged out. 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