货号：	SL100489-L929
保存条件：	4°C
供应商：	济南美中清水湾生物科技有限公司
数量：	现货 大量
英文名：	GenJet™ In Vitro DNA Tranfection Reagent for L929 Cell
保质期：	一年
规格：	毫升/支

DescriptionGenJet™ DNA In Vitro Tranfection Reagent for L929 cell is pre-optimized for transfecting L929 cells. L929 is a murine aneuploid fibrosarcoma cell line which is often used to assay TNF-alpha and TNF-beta. Treatment with TNF initiates apoptosis and subsequent cell death.  The level of detection is on the order of 50 pg/mL. Another cell line which is approximately 5-10-fold more sensitive is WEHI-164. Some L929 subclones have been developed that allow detection of TNF at concentrations of approximately 30 pmol/L (approximately 500 fg/mL). Sublines that are resistant to TNF have been established also. Refer to the following optimal transfection conditions for maximal transfection efficiency on L929 cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 150 to 300 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:Confluence on the day of transfectionCell culture conditionsGenJet™ (µl) : DNA (µg) RatioDiluent for DNA and Transfection ReagentIncubation Time to Form GenJet™/DNA ComplexPresence of Serum/Antibiotics during TransfectionChange Medium After TransfectionMaximal Efficiency Transfection Results:Reporter GenePlasmidEfficiency (GFP %)

~80%DMEM with 4.5 g/L glucose, 10% FBS3:1Serum-free DMEM with 4.5 g/L glucose15 minutes at RTYesYes, ~5 Hours After Transfection48 hoursEGFPpEGFP-N3 (CMV promoter) 65%

Storage ConditionStore at 4 °C. If stored properly, the product is stable for 12 months or longerData Sheet   温馨提示：不可用于临床治疗。