货号：	SL100489-RIN
保存条件：	4°C
供应商：	济南美中清水湾生物科技有限公司
数量：	现货 大量
英文名：	GenJet™ In Vitro DNA Tranfection Reagent for Rin Related Cells
保质期：	一年
规格：	毫升/支

DescriptionGenJet™ DNA In Vitro Tranfection Reagent for Rin is pre-optimized znd conditioned for transfecting Rin and related cells including Rin-14B, Rin-m5F, Rin-m and Rin-5F. Refer to the following optimal transfection conditions for maximal transfection efficiency on Rin related cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 150 to 300 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:Confluence on the day of transfectionCell culture conditionsTransfection MediumGenJet™ (µl) : DNA (µg) RatioDiluent for DNA and Transfection ReagentIncubation Time to Form GenJet™/DNA ComplexPresence of Serum/Antibiotics during TransfectionChange Medium 5 Hours After TransfectionMaximal Efficiency Transfection Results:Reporter GenePlasmidEfficiency (GFP %)

~90%DMEM with 4.5 g/L glucose, 10% FBSDMEM with 4.5 g/L glucose, 1% FBS3:1Serum-free DMEM with 4.5 g/L glucose15 minutes at RTYes in presence of 1% FBSNo48 hoursEGFPpEGFP-N3 (CMV promoter) ~85%

Storage ConditionStore at 4 °C. If stored properly, the product is stable for 12 months or longerA Picture Showing Transfection Efficiency of GenJet™ Reagent on Rin-5F Cells. Rin-5F cells were grown per ATCC recommended culture medium and transfected with pEGFP-N3 by GenJet™. The efficiency was checked 24 hours post transfection Data Sheet   温馨提示：不可用于临床治疗。