货号：	P48-10H-10
保存条件：	–70
规格：	10ug

Description: Recombinant full-length human AMPK (combination of A2/B1/G1 subunits) was expressed by baculovirus in Sf9 insect cells using a C-terminal His tags.      Species: Human      Tag: HIS tags      Expression System: Sf9 insect cells using baculovirus      Sequence: Full Length      Genbank Number: NM_006252  NM_006253  NM_002733      Specific Activity : Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet. Purity : Sample Purity Data. For specific information on a given lot, see related technical data sheet. Storage, Stability and Shipping : Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Applications : Kinase Assay, Western Blot Molecular Weight : ~69kDa (A2), ~38kDa (B1), and ~40kDa (G1). Gene Aliases : Subunits A2: PRKAA2, AMPK, AMPK2, PRKAA Subunit B1: PRKAB1, AMPK, HAMPKb, MGC17785 Subunit G1: PRKAG1, AMPKG, MGC8666 Scientific Background : AMPK (A2/B1/G1) plays a key role in insulin signaling pathway and is a major therapeutic target for the treatment of diabetes (1). AMPK is viewed as a fuel sensor for glucose and lipid metabolism by modulating the activity of the autonomous nervous system in vivo. Short-term overexpression of a constitutively active form of AMPK in the liver leads to mild hypoglycemia and fatty liver due to increased fatty acid utilization (2). References : 1. Viollet, B. et al: Physiological role of AMP-activated protein kinase (AMPK): insights from knockout mouse models. Biochem. Soc. Trans. 2003; 31; 216–219. 2. Foretz, M. et al: Short-term overexpression of a constitutively active form of AMP-activated protein kinase in the liver leads to mild hypoglycemia and fatty liver. Diabetes, 2005; 54 (5);1331-1339. Formulation : 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.  温馨提示：不可用于临床治疗。