Effective March 1, 2018, Merck KGaA, Darmstadt, Germany* is the exclusive Distributor of Avanti Polar Lipids, Inc. Research Products for all countries except the United States. For product pricing and order placement, please click on the appropriate Location Box below.
For customers located outside the United States, you will be redirected to www.sigmaaldrich.com/avanti prior to completing your purchase. Thank you for your continued business and support of Avanti Polar Lipids, Inc!
*The life science business of Merck KGaA, Darmstadt, Germany operates as MilliporeSigma in the U.S. and Canada
Custom Packaging (${custompackaging} @ $4 per aliquot): ${custompackagingperunit|money} Custom packaging is calculated at $4.00 per aliquot + $100.00 surcharge.For all other custom packaging options not listed above, please contact us for a quote![\"Huzzah®](\"https://avantilipids.com/assets/products/images/360492-Web-Structure.gif\")
Huzzah® S1P Human Serum Albumin/Sphingosine-1-Phosphate Complex Avanti’s new HSA lipid delivery system, Huzzah , improves the cellular delivery of S1P through its conjugation with human serum albumin (HSA), a physiologically relevant carrier protein. This conjugate system eliminates the need for organic solvents, which are well-known to adversely affect viability and the phenotypic characteristics of cells.The Huzzah lipid delivery system is supplied as a lyophilized conjugate which can be directly and easily reconstituted in water or a buffer of choice and is ideal for studying the effects of poorly soluble bioactive molecules.Solubility: Reconstitute lyophilized conjugate in 5 mL of water or other aqueous buffer. This produces a stock solution of 120 - 200 µM sphingosine-1-phosphate*. A short sonication may be necessary to ensure complete dissolution. If necessary, filter sterilize the reconstituted conjugate prior to use in cell culture applications.*Exact concentration will vary from batch to batch. Refer to label for exact product concentration upon reconstitution as determined by mass spectrometric analysis.Molar Ratio: S1P:albumin (2:1)Albumin Biological Characterization: microbial free; endotoxin 1 IU/mLAlbumin Lipid Characterization: ~0.07g/L phospholipids; ~0.02 g/L sphingolipids; ~0.1 g/L fatty acids; and triglycerides and cholesterols below the limit of quantitation of 0.1 g/L and 0.05 g/L Del Gaudio I, Hendrix S, Christoffersen C, Wadsack C. Neonatal HDL Counteracts Placental Vascular Inflammation via S1P-S1PR1 Axis. Int J Mol Sci. 2020 Jan 25;21(3):E789. doi: 10.3390/ijms21030789. PMID: 31991780.
PubMed ID: 31991780Del Gaudio I, Sreckovic I, Zardoya-Laguardia P, Bernhart E, Christoffersen C, Frank S, Marsche G, Illanes SE, Wadsack C. Circulating cord blood HDL-S1P complex preserves the integrity of the feto-placental vasculature. Biochim Biophys Acta Mol Cell Biol Lipids. 2020 Apr;1865(4):158632. doi: 10.1016/j.bbalip.2020.158632. Epub 2020 Jan 15. PMID: 31954174.
PubMed ID: 31954174Kang H, Hong Z, Zhong M, Klomp J, Bayless KJ, Mehta D, Karginov AV, Hu G, Malik AB. Piezo1 mediates angiogenesis through activation of MT1-MMP signaling. Am J Physiol Cell Physiol. 2019 Jan 1;316(1):C92-C103. doi: 10.1152/ajpcell.00346.2018. Epub 2018 Nov 14.
PubMed ID: 30427721Leurs CE, Lopes Pinheiro MA, Wierts L, den Hoedt S, Mulder MT, Eijlers AJC, Schoonheim MM, Balk LJ, Uitdehaag BMJ, Killestein J, de Vries HE. Acid sphingomyelinase: No potential as a biomarker for multiple sclerosis. Mult Scler Relat Disord. 2019 Feb;28:44-49. doi: 10.1016/j.msard.2018.11.024. Epub 2018 Nov 30.
PubMed ID: 30553168Zhong L, Jiang X, Zhu Z, Qin H, Dinkins MB, Kong JN, Leanhart S, Wang R, Elsherbini A, Bieberich E, Zhao Y, Wang G. Lipid transporter Spns2 promotes microglia pro-inflammatory activation in response to amyloid-beta peptide. Glia. 2019 Mar;67(3):498-511. doi: 10.1002/glia.23558. Epub 2018 Nov 28.
PubMed ID: 30484906