Eosinophil cationic protein (ECP) is a potent cytotoxic secretory protein with bactericidal and antiviral properties. ECP is released by activated eosinophils and regarded as a marker of eosinophilic inflammation. High levels of ECP have been reported in cases of active asthma and other allergic diseases. Human Periostin ELISA Kit SK00072-08 and Human ECP ELISA Kit SK00128-01 had been used by Dr. Payal K. Naik on follow paper. Periostin promotes fibrosis and predicts progression in patients with idiopathic pulmonary fibrosis Idiopathic pulmonary fibrosis (IPF) is a progressive fibrotic lung disease without effective therapeutics. Periostin has been reported to be elevated in IPF patients relative to controls, but its sources and mechanisms of action remain unclear. We confirm excess periostin in lungs of IPF patients and show that IPF fibroblasts produce periostin. Blood was obtained from 54 IPF patients (all but 1 with 48 wk of follow-up). We show that periostin levels predict clinical progression at 48 wk (hazard ratio = 1.47, 95% confidence interval = 1.03-2.10, P 0.05). Monocytes and fibrocytes are sources of periostin in circulation in IPF patients. Previous studies suggest that periostin may regulate the inflammatory phase of bleomycin-induced lung injury, but periostin effects during the fibroproliferative phase of the disease are unknown. Wild-type and periostin-deficient (periostin(-/-)) mice were anesthetized and challenged with bleomycin. Wild-type mice were injected with bleomycin and then treated with OC-20 Ab (which blocks periostin and integrin interactions) or control Ab during the fibroproliferative phase of disease, and fibrosis and survival were assessed. Periostin expression was upregulated quickly after treatment with bleomycin and remained elevated. Periostin(-/-) mice were protected from bleomycin-induced fibrosis. Instillation of OC-20 during the fibroproliferative phase improved survival and limited collagen deposition. Chimeric mouse studies suggest that hematopoietic and structural sources of periostin contribute to lung fibrogenesis. Periostin was upregulated by transforming growth factor- in lung mesenchymal cells, and periostin promoted extracellular matrix deposition, mesenchymal cell proliferation, and wound closure. Thus periostin plays a vital role in late stages of pulmonary fibrosis and is a potential biomarker for disease progression and a target for therapeutic intervention. Naik PK et al. Am J Physiol Lung Cell Mol Physiol.2012 Dec 15;303(12):L1046-56. Full paper The pooled research mouse EDTA plasma samples, rat EDTA plasma and serum samples were diluted with Dilution Buffer DB08 and assayed by Mouse/Rat Eosinophil cationic Protein (ECP) ELISA Kit SK00128-03 HumanEosinophil Cationic Protein (ECP) ELISA Kit Code No.: SK00128-01 Size: 96 T Standard range:156-10000 pg/ml Sensitivity: 50 pg/ml Sample Type: Serum, EDTA plasma Dilution Factor: 4-8 for plasma samples, 10-20 for serum samples Intra-CV: 4-8% Inter-CV: 8-12% Protocol: PDF HumanEosinophil Cationic Protein (ECP) ELISA KitCode No.: SK00128-06Size: 96 TPrice: Standard range:78-5000 pg/mlSensitivity: 30 pg/mlSample Type: Serum, EDTA plasmaDilution Factor: 40-80 for plasma or serum samplesIntra-CV: 4-8%Inter-CV: 8-12%Protocol: PDFSample Type: Serum, EDTA plasmaDilution Factor: 4-8 for mouse samples; 2 for rat samplesSpecificity: Mouse, ratIntra-CV: 4-8%Inter-CV: 8-12%Protocol: PDF Anti HumanECP Monoclonal AntibodyCode No.: A00128-07-100 Size: 100 ug Price: $359.00 USD Clone:Antigen: human ECPSpecificity: HumanAntibody Type:Purified IgGPurification: Protein G affinityApplication: E, Data Sheet: PDF

Human ECP Recombinant Code No.: 00128-01-100 Size: 100 ug Price: $360.00 USD Protein ID:P12724 Gene ID: 6037 MW:17.3 KD Tag: His Tag on N-Terminus Expressed: E. Coli Purity: 90% Data Sheet: PDF Anti HumanECP IgG Code No.: A00128-01-100 Size: 100 ug Price: $260.00 USD Host:Rabbit Antigen: human ECP Specificity: Human Antibody Type:Purified IgG Purification: Protein A affinity Application: E, IHC, WB Data Sheet: PDF Eosinophil Cationic Protein (ECP) (Human) ELISA Kit SK00128-01 96 T Eosinophil Cationic Protein (ECP) (Human) ELISA KitSK00128-06 96 T Eosinophil Cationic Protein (ECP) (Mouse/Rat) ELISA Kit SK00128-03 96 T Eosinophil Cationic Protein (ECP) (Human) Rec.00128-01-100 100 ug360.00 Eosinophil Cationic Protein (ECP) (Mouse) Rec.00128-03-100 100 ug360.00 Anti Eosinophil Cationic Protein (ECP) (Human) IgG A00128-01-100 100 ug 260.00 Anti Eosinophil Cationic Protein (ECP) (Human) IgG, Biotinylated A00128-01-50B 50 ug 350.00 Anti Eosinophil Cationic Protein (ECP) (Mouse) IgG A00128-03-100 100 ug 260.00 Anti Eosinophil Cationic Protein (ECP) (Mouse) IgG Biotinylated A00128-03-50B 100 ug 260.00 Anti Eosinophil Cationic Protein (ECP) (Human) Monoclonal AntibodyA00128-07-100 100 ug 359.00 Goat Anti Eosinophil Cationic Protein (ECP) (Human) IgG A00128-04-100 100 ug 320.00