Description:

KAPA RNA HyperPrep Kits with RiboErase (HMR)

Single-Day RNA

The KAPA RNA HyperPrep Kits with RiboErase (HMR) utilize novel chemistry that enables the combination of enzymatic steps and fewer reaction purifications, resulting in a truly streamlined solution for the preparation of high-quality rRNA-depleted RNA-seq libraries. By utilizing a targeted enzymatic method for depletion, our workflow enables efficient reduction of ribosomal RNA (rRNA) and a more complete representation of the transcriptome, including precursor mRNAs and non-coding RNA (ncRNA). The strand-specific workflow is flexible – supporting library construction from lower-input amounts and degraded samples. Kits contain all reagents required for rRNA depletion and library preparation, with the exception of KAPA Adapters (available separately). Benefits include:

• single-day library construction, inclusive of rRNA depletion
• reduced hands-on and overall time through fewer enzymatic and reaction cleanups
• flexible input of 25 ng – 1 µg total RNA from human, mouse, or rat species*
• higher success rates with lower input and degraded samples
• maintain over 99% strand specificity*
• KAPA Pure Beads included for reaction purifications

NEW! KAPA Dual-Indexed Adapter Kits are now available. For more information on KAPA Adapter Kits, scroll down to the Ordering section, or download the KAPA Adapter and Bead Calculator. Download our Adapter and Bead Calculator

^{*Data on file.}For Research Use Only. Not for use in diagnostic procedures.

Product Highlights

Sequence what matters

• Waste fewer reads due to the combination of rRNA carryover and PCR duplicates
• Identify more unique transcripts and genes with equivalent sequencing

Achieve superior coverage uniformity

• Obtain more uniform distribution of reads across transcripts
• Improve coverage of difficult GC-rich transcripts

Generate high-quality libraries from degraded samples

• Input as little as 25 ng with FFPE samples, depending on total RNA quality
• Achieve low duplication rates and highly efficient, reproducible rRNA removal with degraded samples
• Identify more unique transcripts and genes with equivalent sequencing

Achieve reliable results with degraded inputs

• Attain a high-degree of expression correlation between paired FFPE and fresh frozen samples which provides increased confidence in sequence data accuracy

Related Products

Are you sequencing low-input, FFPE or high quality DNA?  RNA?  Check out these Kapa NGS products to improve your workflow and results:

KAPA mRNA HyperPrep Kits

KAPA Stranded mRNA-Seq Kits

KAPA Library Amplification Kits

KAPA HyperPrep Kits

KAPA Library Quantification Kits

Applications:

• Whole transcriptome
• Gene expression analysis
• Detection of gene fusions, isoforms, and other structural variants
• Novel transcript identification, including noncoding transcripts
• SNV discovery

Kit Specifications and Contents / Storage:

Enzymes and buffers for rRNA depletion, cDNA synthesis, and library preparation can be stored for up to 10 months at -20°C. KAPA Pure Beads can be stored for up to 10 months at 4°C. (US only)

Kits contain Hybridization Buffer, Hybridization Oligos (HMR), Depletion Buffer, RNase H, DNase Buffer, DNase, Fragment, Prime and Elute Buffer (2X), 1stStrand Synthesis Buffer, KAPA Script, 2nd Marking Buffer, 2nd Strand Synthesis & A-Tailing Enzyme Mix, Ligation Buffer, DNA Ligase, PEG/NaCl Solution, KAPA Pure Beads, Library Amplification Primer Mix (10X), and KAPA HiFi HotStart ReadyMix (2X).

Specifications