Description:

KAPA Library Quantification Kits

for Next-Generation Sequencing

Our standards don’t change. Neither should yours.

KAPA Library Quantification Kits contain all the reagents needed for the accurate, reliable and reproducible qPCR-based quantification of NGS libraries prior to pooling for capture or flow cell amplification. Kits contain KAPA SYBR^® FAST qPCR Master Mix, optimized for high-performance SYBR Green I-based qPCR. The engineered KAPA SYBR FAST DNA Polymerase contained in the Master Mix amplifies GC- and AT-rich DNA fragments of different lengths with similar efficiency, making it the only qPCR Master Mix capable of accurate qPCR-based quantification of NGS libraries.* The pre-diluted set of DNA Standards contained in each kit is carefully quality-controlled to ensure lot-to-lot consistency and avoid data drift over time. Optimized Primer Premixes ensure optimal PCR efficiency.

Kits with DNA Standards and Primer Premixes for Illumina^® and Ion Torrent™ sequencers are available. Please reference the Instrument Compatibility Chart for guidance on compatible platforms.

NEW! Improve experimental designs with our Technical Guide for Illumina Platforms

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For Research Use Only. Not for use in diagnostic procedures.

Product Highlights

Accurately quantify PCR-competent sequencing templates

• qPCR only \"counts” those library molecules that can be sequenced
• More consistent cluster densities or template-to-bead ratios enable optimal utilization of sequencing resources

Improve pooling for multiplexed sequencing

• Library concentrations determined by qPCR are more reliable compared to other methods*
• The engineered KAPA SYBR FAST qPCR Master Mix enables accurate pooling of libraries with extreme GC-contents or longer inserts*

Eliminate drift with carefully quality-controlled DNA Standards

• Prediluted DNA Standards eliminate quantification errors associated with preparation of standard curves using in-house standards*
• KAPA DNA Standards undergo strict quality control to ensure lot-to-lot consistency and eliminate data drift over time

Improve throughput with automation

• Library quantification assay is compatible with 96- and 384-well format
• Library dilution, reaction setup and data analysis can be automated for HTP pipelines
• Learn More about KAPA Automated Solutions

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Related Products

Normalizing libraries for pooling and cluster amplification? Check out these Kapa NGS products to improve your workflow and results:

KAPA hgDNA Quantification and QC Kit

KAPA Library Amplification Kits

KAPA Hyper Prep Kits

KAPA Stranded mRNA-Seq Kits

Applications:

• Quantification of libraries prior to pooling for multiplexed sequencing
• Quantification of individual libraries or library pools prior to cluster amplification or emPCR
• Quantification of libraries prior to and after hybridization capture
• Quality control, optimization and troubleshooting of library construction processes or workflows

Kit Specifications and Contents / Storage:

Kits can be stored for up to 12 months at -20˚C.

Complete kits include KAPA SYBR FAST qPCR Master Mix (2X), Primer Premix (10X) and a set of 6 DNA Standards. Reagents for qPCR (KAPA SYBR FAST qPCR Master Mix and Primer Premix) and DNA Standards (1–6) are also sold separately. Where noted, Master Mixes contain instrument-specific reference dyes, while the Universal kit includes ROX High and ROX Low (both 50X) separately.

Components–Illumina^® Platform

Components–Ion Torrent™ Platform

Specifications