Description:

KAPA mRNA HyperPrep Kits

Single-Day RNA

The KAPA mRNA HyperPrep Kits utilize novel chemistry that enables the combination of enzymatic steps and fewer reaction purifications, resulting in a truly streamlined solution for the preparation of high-quality mRNA-seq libraries. mRNA capture beads are used prior to library preparation, which enriches for mature mRNA over non-polyadenylated species, such as ribosomal, precursor, and noncoding RNAs.

The strand-specific workflow is flexible – supporting library construction from lower-input amounts and degraded samples. Kits contain all reagents required for mRNA capture and library preparation, with the exception of KAPA Adapters (available separately). Benefits include:

• single-day library construction, inclusive of mRNA capture
• reduced hands-on and overall time through fewer enzymatic and reaction cleanups
• flexible input of 50 ng – 1 µg total RNA*
• maintain over 99% strand specificity*
• KAPA Pure Beads included for reaction purifications

KAPA RNA HyperPrep Kits

KAPA RNA HyperPrep Kits leverage the same cDNA synthesis and library preparation workflow improvements as outlined for the KAPA mRNA HyperPrep Kits. KAPA RNA Hyper Prep Kits include all enzymes and buffers required for cDNA library preparation, but do not contain mRNA capture reagents or KAPA Adapters. Kits can be used to prepare libraries from 1 ng – 100 ng of total, poly(A)-selected, or rRNA-depleted RNA.

NEW! KAPA Dual-Indexed Adapter Kits are now available. For more information on KAPA Adapter Kits, scroll down to the Ordering section, or download the KAPA Adapter and Bead Calculator.

Download our Adapter and Bead Calculator

^{*Data on file.}For Research Use Only. Not for use in diagnostic procedures.

Product Highlights

Sequence what matters

• Waste fewer reads due to the combination of rRNA carryover and PCR duplicates
• Identify more unique transcripts and genes with equivalent sequencing

Achieve superior coverage uniformity

• Obtain more uniform distribution of reads across transcripts
• Improve coverage of difficult GC-rich transcripts

Related Products

Are you sequencing low-input, FFPE or high quality DNA?  RNA?  Check out these Kapa NGS products to improve your workflow and results:

KAPA RNA HyperPrep Kits with RiboErase

KAPA Stranded mRNA-Seq Kits

KAPA Library Amplification Kits

KAPA Hyper Prep Kits

KAPA Library Quantification Kits

Applications:

• Gene expression analysis
• Detection of gene fusions, isoforms, and other structural variants
• Novel transcript identification, including noncoding transcripts
• SNV discovery
• Whole transcriptome (KAPA RNA Hyper Prep Kits only)
• Targeted transcriptome (KAPA RNA Hyper Prep Kits only)

Kit Specifications and Contents / Storage:

Enzymes and buffers for rRNA depletion, cDNA synthesis, and library preparation can be stored for up to 10 months at -20°C. mRNA capture reagents and KAPA Pure Beads can be stored for up to 10 months at 4°C. (US only)

Kits include reagents for RNA fragmentation, cDNA synthesis, and library preparation. Kits with reagents for mRNA capture are also available.

Specifications